Sequences from confirmation amplicon


Confirmation sequences for line 155D10 and hit At4g32175 are shown.
Line ID 155D10
Locus At4g32175 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15536987
Primers used for PCR 11vf - 8474, show primer details
   
Sequence Name 24-K117753-0022-155-D10C-11vf
GenBank Accession LN497168 [GenBank]
Sequencing primer gene specific primer, 11vf: 5'-ATTTCTCAACCATGATTCTCTGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K117753-0022-155-D10C-11vf
CATTGGCCACGATATGACTATACGTGGAGCTGTCGCATTTACCCAAACTCTGCCATTTAA
GCCCACAGCTATCTCGAACGAGAGTTTCTTTCCAAGAGCCTCAATGACCGGGCGCGTTGG
TGAATTCAACAGCCTATAAGAGATGAGAAAGTAAAGTTGTAATGGCGAAAGAAGATAGAT
AAAGTATAAGGAGACATTTTTTATGAGGAAGTATATCTCACATTCTTGAAAGACCAGTTG
AAGTTTCAAACATGAAACCATCTCTCAAGGGTCCAAATAATGCAGCTTTCCCACTAGCTA
ATACATAGAAACAAGAGACAATTAGTTTAAAAGGGATTAAGAGAAGCAGCACTGTTTTAG
CCTCCAAAGCTTTTAACTTACCCTCGGTACAAGACAGCTCCGGATTCATTCCTATGTTTG
TCTTGACAACCCGGAGATAAAGCAGAGTGCCTACCTGTTTAGTACACAAAGAGTATGGCT
TCATGTAATTACCTGTAGATAAAGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATT
   
Sequence Name 08-K117752-0022-155-D10C-8409
GenBank Accession LN489915 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K117752-0022-155-D10C-8409
ATCCTGCTTTATCTACAGGTAATTACATGAAGCCATACTCTTTGTGTACTAAACAGGTAG
GCACTCTGCTTTATCTCCGGGTTGTCAAGACAAACATAGGAATGAATCCGGAGCTGTCTT
GTACCGAGGGTAAGTTAAAAGCTTTGGAGGCTAAAACAGTGCTGCTTCTCTTAATCCCTT
TTAAACTAATTGTCTCTTGTTTCTATGTATTAGCTAGTGGGAAAGCTGCATTATTTGGAC
CCTTGAGAGATGGTTTCATGTTTGAAACTTCAACTGGTCTTTCAAGAATGTGAGATATAC
TTCCTCATAAAAAATGTCTCCTTATACTTTATCTATCTTCTTTCGCCATTACAACTTTAC
TTTCTCATCTCTTATAGGCTGTTGAATTCACCAACGCGCCCGGTCATTGAGGCTCTTGGA
AAGAAACTCTCGTTCGAGATAGCTGTGGGCTTAAATGGCAGAGTTTGGGTAAATGCGACA
GCTCCACGTATAGTCATTATCGTGGCCAATGCAATAATGAACTCGGAATCTCTAAGTGGG
ACTCAGCAGAGAATCA

Last Updated on 06.12.2018 16:11