Sequences from confirmation amplicon


Confirmation sequences for line 156G02 and hit At5g58430 are shown.
Line ID 156G02
Locus At5g58430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23621930
Primers used for PCR NK16 - 8474, show primer details
   
Sequence Name 14-K102588-0022-156-G02C-8409
GenBank Accession LN492457 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K102588-0022-156-G02C-8409
TATCCTGATGAATAATGGGAGATACATTGCTCTGAAAGTCAAAGATGGAGACCTAGGATT
GCTGTTAGGAGATGATTGGATTCGGAAACACAATGTCAAAGTGAAACAATACCATATGAA
TTATCAGAGAAGCTCATGGAATAAGATGCTGGGGTTGCTGAAGGTGGATAATACTGCAGC
AGGCATGAACGGTTTGGGGAAGACCATGAAGGAGAAGCTGAAGCAGTTCAATATTCAGTT
TGATGAGATATGTAAAGTGCATTCGACGTGGGTAGTGTTTGACGAGCAGCTGAAAGAAGA
GCTAAAGATCTCACTGGCCAGGCTTTTGGTACCGGCATACGGGAGCTTCATCGGCAGGTT
CCAAAACCTTGGAGACATAGGGAAAAACGCAGACAAGTACATCAAGTATGGAGTTGAGGA
CATAGAGGCGCGTATTAACGAGCTGTTCAAAGGGACTACCACGGGAAGAAAATGAAGGTA
ACGAACACAAAAATAGCCTCTTGTAACATCTTCGTAACGAACCACCTGCTAAATGACCAA
TAGCGAAGTCTTGCAGGGAGG
   
Sequence Name 14-K102589-0022-156-G02C-NK16
GenBank Accession LN492458 [GenBank]
Sequencing primer gene specific primer, NK16: 5'-ATACATGTTACAAATCCTCCCTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K102589-0022-156-G02C-NK16
GTTCGTTCGAAGATGTTACAAGAGGCTATTTTTGTGTTCGTTACCTTCATTTTCTTCCCG
TGGTAGTCCCTTTGAACAGCTCGTTAATACGCGCCTCTATGTCCTCAACTCCATACTTGA
TGTACTTGTCTGCGTTTTTCCCTATGTCTCCAAGGTTTTGGAACCTGCCGATGAAGCTCC
CGTATGCCGGTACCAAAAGCCTGGCCAGTGAGATCTTTAGCTCTTCTTTCAGCTGCTCGT
CAAACACTACCCACGTCGAATGCACTTTACATATCTCATCAAACTGAATATTGAACTGCT
TCAGCTTCTCCTTCATGGTCTTCCCCAAACCGTTCATGCCTGCTGCAGTATTATCCACCT
TCAGCAACCCCAGCATCTTATTCCATGAGCTTCTCTGATAATTCATATGGTATTGTTTCA
CTTTGACATTGTGTTTCCGAATCCAATCATCTCCTAACAGCAATCCTAGGTCTCCATCTT
TGACTTTCTGAACAATGTATCTCCCATTATTCATCAGGATATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATA
AAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAG
CAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACTCCCA
AAATGG

Last Updated on 06.12.2018 16:11