Sequences from confirmation amplicon


Confirmation sequences for line 156H02 and hit At2g03933 are shown.
Line ID 156H02
Locus At2g03933 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:1217792
Primers used for PCR 04ou - 8474, show primer details
   
Sequence Name 17-K115635-0022-156-H02C-04ou
GenBank Accession LN493990 [GenBank]
Sequencing primer gene specific primer, 04ou: 5'-CATGCCATACATGCCAATCTATAA-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K115635-0022-156-H02C-04ou
TATCAACAAATACGGAAAATTTCATTTATGTTAAAAAAAGAGTAATCCTACTATGTTTGA
AAAACTAAGAACTTCTCTCAACAAAGAATTATATAAATATAGCTTTCTGTAAAGGCTTAG
AGAAGTAGAATTTCTTTTCTTTTTAAAATAAGACTTTTTATTTTTTGAAAAAATTAACAG
CAAAAAAAAATGAATATCACAAAAAGTTATGTGGTGATTTTTTTTCTAGTGATGTTAACA
AATTCATTGTCCAACTCCGACATTTTGGTTTCATCAGGTATGTTGTTACTATTCAATATC
TTTGATTATTTTAAAAGTAACTATTGAATAGTTTACTTTTTAAAATCGCTTTTAATGTAT
ATGTTTGCGTATTTATATTACTTTTCTTTAACATAAATTGTATATGTATTGTAGATACAT
CATGATATGATGGCTTCATATATTCAATTGAATGAGTATTACGTAATATATCAGGATATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATT
CC
   
Sequence Name 03-K115634-0022-156-H02C-8409
GenBank Accession LN487620 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K115634-0022-156-H02C-8409
ATATTACGTAATACTCATTTCAATTGAATATATGAAGCCATCATATCATGATGTATCTAC
AATACATATACAATTTATGTTAAAGAAAAGTAATATAAATACGCAAACATATACATTAAA
AGCGATTTTAAAAAGTAAACTATTCAATAGTTACTTTTAAAATAATCAAAGATATTGAAT
AGTAACAACATACCTGATGAAACCAAAATGTCGGAGTTGGACAATGAATTTGTTAACATC
ACTAGAAAAAAAATCACCACATAACTTTTTGTGATATTCATTTTTTTTTGCTGTTAATTT
TTTCAAAAAATAAAAAGTCTTATTTTAAAAAGAAAAGAAATTCTACTTCTCTAAGCCTTT
ACAGAAAGCTATATTTATATAATTCTTTGTTGAGAGAAGTCTTAGTTTTTCAACATAGTA
GATTACTCTTTTTTTAACATAAATGAATTTTCCGTATTTGTTGATAACATTATTTTGGTG
GTAAGAAAATATCAACATATAGTTATAGATT

Last Updated on 06.12.2018 16:11