Sequences from confirmation amplicon


Confirmation sequences for line 157B01 and hit At5g59845 are shown.
Line ID 157B01
Locus At5g59845 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24111769
Primers used for PCR RP05 - 8474, show primer details
   
Sequence Name 27-K023494-022-157-B01C-8409
GenBank Accession LN498206 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K023494-022-157-B01C-8409
CCGGACATGAAGCCATTTACTCTTTCTTGTTGCTGATCATTGCGGTAACAAGGGCATTCA
TCTTTGTTTCCATAAGTGCCTGAAGGAACACAATAGTTACACTTCTCGCAACATATATTA
CAATACTTGAGACACCTATCTTGTCTTCCTGCCTTTGAACATCTCACGTTGCATTTTCCT
CCGCATGGTGCTAATTAATCCATCATCACAATACAATTTTATTAAGAAAGCTAAATTTCT
AATGAGAAAAGTATCTAAAATAAGAAAATGCATTGTGTATACTTACACGAATCCGCGGTT
GAGAGTATGAACAAAGAAGATGTGATGAGAAGAGAGATAATAAGAACTTTTACAGCCGGG
AATTTCATCGTAGTTCTTGATGAGATTGTTTTAATAATAATCTGTTTGGAGAAAGTTTGT
TACTTTGAGAAGATGAAGAGAAAACTCGAAGGTTTCACACAATGCAAAAGGAGTGATGGT
TTGGGAAGAAAAGGAGGTTACAAAAAGAGTTACTTATAGGGAAATTTTGGAGTAGGTGAA
ACGCTTGTTATCTAGTCCATCTGTCCCAATTTATAAGATTTTTGTGGGGCTT
   
Sequence Name 28-K023494-022-157-B01C-RP05
GenBank Accession LN498675 [GenBank]
Sequencing primer gene specific primer, RP05: 5'-TTAATCTTTGAACTTAAGCCCCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K023494-022-157-B01C-RP05
AAAATCTTATAAATTGGGACAGATGGACTAGATAACAAGCGTTTCACCTACTCCAAAATT
TCCCTATAAGTAACTCTTTTTGTAACCTCCTTTTCTTCCCAAACCATCACTCCTTTTGCA
TTGTGTGAAACCTTCGAGTTTTCTCTTCATCTTCTCAAAGTAACAAACTTTCTCCAAACA
GATTATTATTAAAACAATCTCATCAAGAACTACGATGAAATTCCCGGCTGTAAAAGTTCT
TATTATCTCTCTTCTCATCACATCTTCTTTGTTCATACTCTCAACCGCGGATTCGTGTAA
GTATACACAATGCATTTTCTTATTTTAGATACTTTTCTCATTAGAAATTTAGCTTTCTTA
ATAAAATTGTATTGTGATGATGGATTAATTAGCACCATGCGGAGGAAAATGCAACGTGAG
ATGTTCAAAGGCAGGAAGACAAGATAGGTGTCTCAAGTATTGTAATATATGTTGCGAGAA
GTGTAACTATTGTGTTCCTTCAGGCACTTATGGAAACAAAGATGAATGCCCTTGTTACCG
CAATGATCAGCAACAAGAAAGAGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGAC
AAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAA
TCT

Last Updated on 06.12.2018 16:11