Sequences from confirmation amplicon


Confirmation sequences for line 157B08 and hit At3g47600 are shown.
Line ID 157B08
Locus At3g47600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17540742
Primers used for PCR 08mv - 8474, show primer details
   
Sequence Name 17-K114626-0022-157-B08C-08mv
GenBank Accession LN493956 [GenBank]
Sequencing primer gene specific primer, 08mv: 5'-CTTCTCTCCCATTGACCTTTACTG-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K114626-0022-157-B08C-08mv
GGAGAAGTCTTTATTATCAAGGCCATTGTTGATAGTACTATCACAAGAATCATTCATCTT
CTTGAGCTTTTTCTTCAAATGAGTGTTCCAATAGTTCTTTATATCATTGTCTGTCCTTTC
TGGAAGATATGATGCTATAGCTGCCCACCTTCATAATTATTTGGTCCCCAAGTTATTTAA
AGTTCAAAACCTTATCTTTATGCATACCCAAAAAAATAAAAGAATTTGTATACTTTTATA
GATCATGTGCATGTGAATATGTATGTATTTACCTGTTTCCCAAAAGAGCTTGAAGATGGA
GAATCATCTTCTCTTCATGCTCGGTGAAATTTCCACGCTTGATCCCAGGTCGAAGATAAT
TAGTCCACCTCAATCTACAGCTTTTGCTACATCTCCTCAAACCTAAATCACCACAACAAC
AACAAGATAATTTCATTAAAAGAGTAATTAATTTGTGATATATGGACAAAAAAGCTGGTG
CCATAAATCGGATATTATTACAATTTTTCCTTTTCATTAAATGATCATACCAACCAAGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAG
   
Sequence Name 17-K114625-0022-157-B08C-8409
GenBank Accession LN493955 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K114625-0022-157-B08C-8409
ATCATTTATGAAAGGAAAAATTGTAATAATATCCGATTTATGGCACCAGCTTTTTTGTCC
ATATATCACAAATTAATTACTCTTTTAATGAAATTATCTTGTTGTTGTTGTGGTGATTTA
GGTTTGAGGAGATGTAGCAAAAGCTGTAGATTGAGGTGGACTAATTATCTTCGACCTGGG
ATCAAGCGTGGAAATTTCACCGAGCATGAAGAGAAGATGATTCTCCATCTTCAAGCTCTT
TTGGGAAACAGGTAAATACATACATATTCACATGCACATGATCTATAAAAGTATACAAAT
TCTTTTATTTTTTTGGGTATGCATAAAGATAAGGTTTTGAACTTTAAATAACTTGGGGAC
CAAATAATTATGAAGGTGGGCAGCTATAGCATCATATCTTCCAGAAAGGACAGACAATGA
TATAAAGAACTATTGGAACACTCATTTGAAGAAAAAGCTCAAGAAGATGAATGATTCTTG
TGATAGTACTATCAACAATGGCCTTGATAATAAAGACTTCTCCATATCAAACAAAAACAC
TACCTCACATCANAGCAGCAACTCCNGTAAAGGTCAAT

Last Updated on 06.12.2018 16:11