Sequences from confirmation amplicon


Confirmation sequences for line 157C10 and hit At1g07790 are shown.
Line ID 157C10
Locus At1g07790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2413485
Primers used for PCR HF33 - 8474, show primer details
   
Sequence Name 31-K032415-022-157-C10C-8409
GenBank Accession LN500047 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K032415-022-157-C10C-8409
TTACAATTGAATATATACCCGGTTAGGGTTTTTAGGGATTTCAATTTCGGTTTGGTTTTT
AGTACTTGTAGGAAATTAGATGCATTATTCGCTTAACTTCGTATTTTGTGGCTTTGTAAC
TCTAGGAAGTAGAGAAGATTGTTCCTTCCTTTTATGAATTTCAATGTTGGATTATATATT
TTTAACGATTTTTTTTTCCATTTCAATTTCATTTACCTAATCACTATATTGAAATGTGTC
TCTATTGAAGTATATGTTATATGTGTGTGTTTATATTATTTGATACGCGAAATTGGCCTA
CTTTTCCTTGAATGATGTTGCTTTCTTACCTTCTTATAACGAACAGAGTCTAATTATGAT
ATGATTGGAGTCATTGAAATAGATAAAATTGAAGATTAGTAGTTGAATTACCTATC
   
Sequence Name 31-K032415-022-157-C10C-HF33
GenBank Accession LN500048 [GenBank]
Sequencing primer gene specific primer, HF33: 5'-GGCACAACGATAACGATAGGTAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K032415-022-157-C10C-HF33
TTCATGACTCCATCATATCATAATTAGACTCTGTTCGTTATAAGAAGGTAAGAAAGCAAC
ATCATTCAAGGAAAAGTAGGCCAATTTCGCGTATCAAATAATATAAACACACACATATAA
CATATACTTCAATAGAGACACATTTCAATATAGTGATTAGGTAAATGAAATTGAAATGGA
AAAAAAAATCGTTAAAAATATATAATCCAACATTGAAATTCATAAAAGGAAGGAACAATC
TTCTCTACTTCCTAGAGTTACAAAGCCACAAAATACGAAGTTAAGCGAATAATGCATCTA
ATTTCCTACAAGTACTAAAAACCAAACCGAAATTGAAATCCCTAAAAACCCTAACCGGGT
ATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGAT
CCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTC
GACGTGTCTACTTCA
Primers used for PCR HF34 - 8474, show primer details
   
Sequence Name 62-K032419-022-157-C10C-8409
GenBank Accession LN510670 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K032419-022-157-C10C-8409
TTACAATTGAATATACTCATTTGCCGCCATCGGCTCGCCGGCAGAACTTGAGTACGACAG
CAAAAATACTGACAGGTGGTAAACTTGGTAACCGCCTTGGTACCTTCAGAGACGGCGTGT
TTAGCGAGTTCACCGGGGAGGACGAGTCTCACAGCCGTCTGGATCTCGCGAGATGTAATG
GTCGGCTTCTTGTTGTACCTAGCGAGTTTCGAAGATTCCTGAGCTAGCTTCTCGAATATG
TCGTTGATGAAACTGTTCATAATCCCCATGGCTTTGCTTGAAATTCCGATATCTGGATGA
ACCTGCTTCAGCACCTTGAAGATGTAGATCTTGTAGGTTTCGACGTTTTTCTTAGATCGC
TTCTTCTTCTTGTCACCGGCTTCCTTTGGTGGCAATTTCTTCCCCGCCTTGGGTTTCTTC
TCCGCCGGAGCCTTTTCAGCAGCCTTGTTCTCTTCCACCGGTCTCTCAGCGGCGGTTTTT
TTCTCGGCGGGCTTCTTCTCGGCTCTTGGTGCCATCGTGAATTT
   
Sequence Name 62-K032419-022-157-C10C-HF34
GenBank Accession LN510671 [GenBank]
Sequencing primer gene specific primer, HF34: 5'-AATCAGAAAGAAAATTCACGATGG-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K032419-022-157-C10C-HF34
CGCCGAGAAAAACCGCCGCTGAGAGACCGGTGGAAGAGAACAAGGCTGCTGAAAAGGCTC
CGGCGGAGAAGAAACCCAAGGCGGGGAAGAAATTGCCACCAAAGGAAGCCGGTGACAAGA
AGAAGAAGCGATCTAAGAAAAACGTCGAAACCTACAAGATCTACATCTTCAAGGTGCTGA
AGCAGGTTCATCCAGATATCGGAATTTCAAGCAAAGCCATGGGGATTATGAACAGTTTCA
TCAACGACATATTCGAGAAGCTAGCTCAGGAATCTTCGAAACTCGCTAGGTACAACAAGA
AGCCGACCATTACATCTCGCGAGATCCAGACGGCTGTGAGACTCGTCCTCCCCGGTGAAC
TCGCTAAACACGCCGTCTCTGAAGGTACCAAGGCGGTTACCAAGTTTACCACCTGTCAGT
ATTTTTGCTGTCGTACTCAAGTTCTGCCGGCGAGCCGATGGCGGCAAATGAGTATATTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTAT
TTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTG

Last Updated on 06.12.2018 16:11