Sequences from confirmation amplicon


Confirmation sequences for line 157D06 and hit At3g20370 are shown.
Line ID 157D06
Locus At3g20370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7106910
Primers used for PCR PC57 - 8474, show primer details
   
Sequence Name 74-K103762-0022-157-D06C-PC57
GenBank Accession LN513061 [GenBank]
Sequencing primer gene specific primer, PC57: 5'-CCAGGCTTCATCTAGTGTATTGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K103762-0022-157-D06C-PC57
TCAGTTGAGATTAAGATTAGCAATTATATTATTTTATTGTTTATGGCAATACATTAGTGA
TAGTAATTTAGCAGTAGACTCTAACCTCCATCATAAATTATCCATTAATTCTTTAATTCT
TAGGTATCAAAACAAACAAAACTGAGCCGAATGTTGGATTGGCGGATTTTTTTTTGGGTG
AAGACTATTTGATCTTTCAAGAAGATATATGATAATTTGATAATGATATGCTGAAGAAAA
AGAAACAATTGTATTTGAAATGCATAATCAAATTCTTATTAGTATTTGATAACAAATCAA
ACACATGGAGGTAGGTACAAGTTCTTTGCTTAGAGAAAATCTAACTCGGGAAGTACTTGG
TTGAAGAAATGGCCTCCATTTCAACTTGAACCATCAACACATCGTTCTTAACGAAACCCT
TTGATGAATCTGTGAGATAAGGGAAATAGATAAACTCAGGGAAACCCCAGCTATATTCTC
CATATCCCGGACCATTGTACCAAATATCGATGGCTTCCAGGATATATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATT
   
Sequence Name 65-K103763-0022-157-D06C-8409
GenBank Accession LN511386 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K103763-0022-157-D06C-8409
ATTTACAATTGAATATATCCTGGAAGCCATCGATATTTGGTACAATGGTCCGGGATATGG
AGAATATAGCTGGGGTTTCCCTGAGTTTATCTATTTCCCTTATCTCACAGATTCATCAAA
GGGTTTCGTTAAGAACGATGTGTTGATGGTTCAAGTTGAAATGGAGGCCATTTCTTCAAC
CAAGTACTTCCCGAGTTAGATTTTCTCTAAGCAAAGAACTTGTACCTACCTCCATGTGTT
TGATTTGTTATCAAATACTAATAAGAATTTGATTATGCATTTCAAATACAATTGTTTCTT
TTTCTTCAGCATATCATTATCAAATTATCATATATCTTCTTGAAAGATCAAATAGTCTTC
ACCCAAAAAAAAATCCGCCAATCCAACATTCGGCTCAGTTTTGTTTGTTTTGATACCTAA
GAATTAAAGAATTAATGGATAATTTATGATGGAGGTTAGAGTCTACTGCTAAATTACTAT
CACTAATGTATTGCCATAAACAATAAAATAATATAATTGCTAATCTTAAATCTCAACTTG
ACTATAAAGATAAAGACTAAATCGATCAAAAACCAATACACTAGATGAAGCCTGG

Last Updated on 06.12.2018 16:11