Sequences from confirmation amplicon


Confirmation sequences for line 157F08 and hit At4g15740 are shown.
Line ID 157F08
Locus At4g15740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8965225
Primers used for PCR 33pm - 8474, show primer details
   
Sequence Name 27-K115400-0022-157-F08C-33pm
GenBank Accession LN498527 [GenBank]
Sequencing primer gene specific primer, 33pm: 5'-TTGATGAGAAGTCAGCTCAAGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K115400-0022-157-F08C-33pm
CGGCCTTTCCTTGGTGATAGGAGATTGGATTTGTTAGACTTCCGATGCAACAGTTATTGG
TCTCAAATCCGCCTTATCCGTTGACCGACAATGGTGATGCTAACGGTATGAAGTTGGAGA
CACATGCTTTGACGGGTCCTTATGGAAAAAAAGGTGTCGTGAGTATGAGTATGATATATT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGCAGCGATTATTAT
   
Sequence Name 03-K115399-0022-157-F08C-8409
GenBank Accession LN487615 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K115399-0022-157-F08C-8409
TTACATTGAATATATCATACTCATACTCACGACACCTTTTTTTCCATAAGGACCCGTCAA
AGCATGTGTCTCCAACTTCATACCGTTAGCATCACCATTGTCGGTCAACGGATAAGGCGG
ATTTGAGACCAATAACTGTTGCATCGGAAGTCTAACAAATCCAATCTCCTTATCACCAAG
GAAAGGCCGGTGGCTCATCAACTCCACAAGGAGCATCAACCGCCCTTCTTGAGCTGACTT
CTCATCA

Last Updated on 06.12.2018 16:11