Sequences from confirmation amplicon


Confirmation sequences for line 158H06 and hit At5g05870 are shown.
Line ID 158H06
Locus At5g05870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1767785
Primers used for PCR 33ze - 8474, show primer details
   
Sequence Name 18-K124218-0022-158-H06C-33ze
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 33ze: 5'-CGTTTACAAATTGGTCCCACTTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K124218-0022-158-H06C-33ze
CTCTAATGTCGAGTTCCATCCATTATGAGTCAAAAACCCTCCCGTGGCTCTATGCGCAAG
AACGTCTAGCTGCGGTGCCCATCTCACTATCTTTCCTTTACCATCGAGACTTTCCATGAA
CCCTGAAGGTAATGATTCGATCCAATCTCTGCCATGGACTGAACCAGGCCGGACAACCCA
CAAGAAGGATTGGTTGGTGTTTCTTAGTCCACAAGCAATCTCCAAGAAGTCAGACTCGTT
AAGACTCGCAATGCTCCCTAAGCTCACGTAGACTACTGATCTCGTTTCACGCATATCTAA
CCATGGAATGCAACTCTGGTCCGGTTCTAACAAGCTGCTAGACGAGGCTGGGACGTCATG
AATGTGAAAAGGGCCAATGGGAAATATCGGAATGCTGAAAACTTTGTTGGACTCAGCAAG
TGAATCATGGTCAAGCTCTTTGCAGGACATAACTATAATCCCTGAAGCTGGCTTCGTCGC
GTCGAGTATCTTAAGCAAGTAAGCATCTAGAGGCTTACTCTGAGCGCTGGTTCCCATAAT
TCTCGAAAGATCTTTCTTTCGAAGCGGTGGAAACTCAGGAACTAGATCATCTGCCTCCGA
ATCTGAAACAAACCTAGAAAGTTCAAGAAATACGGACCAATTGTGTCTTTGATGACTCAA
TTAACAATAAGAACAATGACCTACTATGAGTCAAAGCCGTACCTGGTACTGGAAGAAACC
CTTCACGACGAATCTGAGGAACAAGAAAATGTCCGAGAAAGAAAGAGAACTTATAAGCAC
AGAGGACAAATCGAGGAAGATTAAAACTCTCCGCCACGGATTGTGTGAAAACCCAACCGG
AATCATCGATCACACAGCTAATTTTCCTATCCTCTGTTCCTGAATCTGAACTAGGTTTAA
TGAGTTTAGCCAAACACTCT
   
Sequence Name 04-K124217-0022-158-H06C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K124217-0022-158-H06C-8409
TCATTGAATATATCCTGATCTACATGCAAGCTCCACCGGGTGCAAAGCGGCAGCGTGTCA
ATTATATGCTTCGGGGACGTGAATATTTCCCGGGGAATGGTTTGAGATTTTCGATCACCA
TCATCCACACGCGCTTCAACGCGCCCAAATCTTCAGACCATCCTCTCTTCACTTTCTTAC
AAATCCGCGACGGCTTGTCTGAATCTCAGACTCAATCTCGTGATCTTTTGCTTCAACTCA
CGCTTCTCAACAACAATTGTCAGATCCCATTTCGAGAGTGTTTGGCTAAACTCATTAAAC
CTAGTTCAGATTCAGGAACAGAGGATAGGAAAATTAGCTGTGTGATCGATGATTCCGGTT
GGGTTTTCACACAATCCGTGGCGGAGAGTTTTAATCTTCCTCGATTTGTCCTCTGTGCTT
ATAAGTTCTCTTTCTTTCTCGGACATTTTCTTGTTCCTCAGATTCGTCGTGAAGGGTTTC
TTCCAGTACCAGGTACGGCTTTGACTCATAGTAGGTCATTGTTCTTATTGTTAATTGAGT
CATCAAAGACACAATTGGTCCGTATTTCTTGAACTTTCTAGGTTTGTTTCAGATTCGGAG
GCAGATGATCTAGTTCCTGAGTTTCCACCGCTTCGAAAGAAAGATCTTTCGAGAATTATG
GGAACCAGCGCTCAGAGTAAGCCTCTAGATGCTTACTTGCTTAAGATACTCGACGCGACG
AAGCCAGCTTCAGGGATTATAGTTATGTCCTGCAAAGAGCTTGACCATGATTCACTTGCT
GAGTCCAACAAAGTTTTCAGCATTCCGATATTTCCCATTGGCCCTTTTCACATTCATGAC
GTCCCAGCCTCGTCTAGCAGCTTGTTAGAACCGGACCAGAGTTGCATTCCATGGTTAGAT
ATGCGTGAAACGAGATCAGTAGTCTA

Last Updated on 06.12.2018 16:11