Sequences from confirmation amplicon


Confirmation sequences for line 158H12 and hit At4g08770 are shown.
Line ID 158H12
Locus At4g08770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:5600019
Primers used for PCR 04ak - 8474, show primer details
   
Sequence Name 21-K106739-0022-158-H12C-04ak
GenBank Accession LN495678 [GenBank]
Sequencing primer gene specific primer, 04ak: 5'-ACCTTGGCCTATAAAAAGAATCGT-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K106739-0022-158-H12C-04ak
AACCAATAATTCTCTTAAAGAGCTAAGAAAATGCATTCCTCTCTGATAAAATTGGGATTT
CTTCTTCTTCTTATTCAGGTATCATTGTCTCATGCTCAACTAAGCCCTTCCTTTTACGAT
AAAACATGTCCGCAAGTCTTTGACATTGCAACCACTACCATTGTCAATGCTCTAAGATCG
GACCCTCGCATCGCTGCAAGCATCCTTCGTCTTCATTTCCACGACTGCTTTGTTAATGTA
AGATACCATTAGAATCAATATATTCATGTATATTCAGGATATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCG
   
Sequence Name 05-K106738-0022-158-H12C-8409
GenBank Accession LN488370 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K106738-0022-158-H12C-8409
TCGGACTGAGCCTTTACATTGAATAATCCTGAATATACATGAATATATTGATTCTCCTTG
TATCTTACATTAACAAAGCAGTCGTGGAAATGAAGACCAATGATGCTTGCAGCGATGCGA
GGGTCCGATCTTAGAGCATTGACAATGGTAGTGGTTGCAATGTCAAAGACTTGCGGACAT
GTTTTATCGTAAAAGGAAGGGCTTAGTTGAGCATGAGACAATGATACCTGAATAAGAAGA
AGAAGAAATCCCAATTTTATCAAAGAGGAATGCATTTTCTTAGCTCTTTTAAGAGAATTA
TTTGGTTATGGTTTAATTTGAGTGAATGTAGGTTTGTCTTAACGATTCTTTTTATAGGCC
AAGGT

Last Updated on 06.12.2018 16:11