Sequences from confirmation amplicon


Confirmation sequences for line 159B12 and hit At2g42950 are shown.
Line ID 159B12
Locus At2g42950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17864520
Primers used for PCR 10cv - 8474, show primer details
   
Sequence Name 23-K109134-0022-159-B12C-10cv
GenBank Accession LN496591 [GenBank]
Sequencing primer gene specific primer, 10cv: 5'-ATGGAGATTCGTCTCTCAGAATGT-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K109134-0022-159-B12C-10cv
ATTAGTGCTTAGTCTCTTTTTTTCTTTTTCTTGTAGAGTGTTTTTTTAACCATTGTTGTT
GTTGTTGTGATAGAAAGAAACAGGAATGTGAAACAGATCAATTTGGTACTGGTGAAGACG
AAGAACATTCTAGGAGTTATTGGCGAGGAATTGGATGGGATAGGATCTCTGAGCTTGTTC
AGACTGTTCAAGTTGATAATAATTGGGATTTGCGAAAGATTGACTTAGATGAGGATGAAG
CCACTGTTGCTGAGCTTGCTGCTCCTTACTGGGAACGACCACTTGCTGGTCCAACTTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGT
TAT
   
Sequence Name 07-K109133-0022-159-B12C-8409
GenBank Accession LN489311 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K109133-0022-159-B12C-8409
TCGGACTGAGCATTTACAGTTGGACCAGCAAGTGGTCGTTCCCAGTAAGGAGCAGCAAGC
TCTGCAACAGTGGCTTCATCCTCATCTAAGTCAATCTTTCGCAAATCCCAATTATTATCA
ACTTGAACAGTCTGAACAAGCTCAGAGATCCTATCCCATCCAATTCCTCGCCAATAACTC
CTAGAATGTTCTTCGTCTTCACCAGTACCAAATTGATCTGTTTCACATTCCTGTTTCTTT
CTATCACAACAACAACAACACTGTTTAAAAAAACACTCTACAAGAAAAAGAAAAAAAGAG
ACTAAGCACTAATATGTATCAACAAGTTAGTAACAATCATCATACATTCTGAGAGACGAA
TCTCCA

Last Updated on 06.12.2018 16:11