Sequences from confirmation amplicon


Confirmation sequences for line 160E03 and hit At3g28590 are shown.
Line ID 160E03
Locus At3g28590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:10718507
Primers used for PCR ML56 - 8474, show primer details
   
Sequence Name 57-K100821-0022-160-E03C-8409
GenBank Accession LN509350 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K100821-0022-160-E03C-8409
GCCATTTACATTGAAAAATAAGGAAATAATGCAGATTTCCTTATTTTCCTTATTTTACAA
TTGCTGATCCATGTAGATTTCCATGGTGATGATAACTGTGGCGGTGCCGGTGGTAATAGT
GAAACAACAGTGGAGGTGGCGATGGTGGCGATGGTGACGATAACAGTGCCGGTGCCGGTG
GTGATGGTGACGGCAACAATGGCGGTACCGGTGACGATGGTGACGACAATAATGGTGGTG
CCGGTGGCGAAAAGATAATTACAAACACCTATTGATCTAGAAAAACTAAACCCTAAGATA
CTATAAATGGAACCCAAACAAAAATCATTAAACGAAATGAAATTATCTCAAGTTTCAATT
TTCAGAATCCTAGCAGC
   
Sequence Name 57-K100833-0022-160-E03C-ML56
GenBank Accession LN509352 [GenBank]
Sequencing primer gene specific primer, ML56: 5'-TGCTGCTAGATTTCTGAAAATTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K100833-0022-160-E03C-ML56
TATGATTTTTGTTTGGGTTCCATTTATAGTATCTTAGGGTTTAGTTTTTCTAGATCAATA
GGTGTTTGTAATTATCTTTTCGCCACCGGCACCACCATTATTGTCGTCACCATCGTCACC
GGTACCGCCATTGTTGCCGTCACCATCACCACCGGCACCGGCACTGTTATCGTCACCATC
GCCACCATCGCCACCTCCACTGTTGTTTCACTATTACCACCGGCACCGCCACAGTTATCA
TCACCATGGAAATCTACATGGATCAGCAATTGTAAAATAAGGAAAATAAGGAAATCTGCA
TTATTTCCTTATTTTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCG

Last Updated on 06.12.2018 16:11