Sequences from confirmation amplicon


Confirmation sequences for line 160G05 and hit At5g20320 are shown.
Line ID 160G05
Locus At5g20320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6860064
Primers used for PCR AK11 - 8474, show primer details
   
Sequence Name 27-K020068-022-160-G05C-AK11
GenBank Accession LN498184 [GenBank]
Sequencing primer gene specific primer, AK11: 5'-AAGAACTGATTGAACTTTGCCAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K020068-022-160-G05C-AK11
AAGTGGGATCGGGAAATATCAGCGACGAAAAAGGATGGTGCTTTTACTGTTGAACTAAAA
GTGACCAAGAATGGTTGTTGCCTTACAGTTTCTGCAACTGGTCGGAACAAAAGAGAGGGC
ACAAAAAAGGCTGCACAGCTGATGATTACAAACCTGAAGGTACTTTCTCAAAATCAAAAG
AAACTGTAGTGTAACAAATTTCTTCAATTGATGTGATGTATATATATCTTAAGCTATCTA
GTGTTCATATATGTAACTCGTTGTTCAAGAACTTCTTTGTGTAATTCATCCGCAGGCTCA
TGAGAACATAACAACCTCCCATCCGTTGGAGGATGTTCTGAAGAATGGCATCCGAAATGA
AGCTAAATTAATTGGCTACAATGAAGATCCTATAGATGTTGTGGATCTTGTTGGGCTGGA
CGTTGAAAACCTAAATATCCTAGAAACTTTTGGCGGGAATAGTGAAAGAAGCAGCTCATA
CGTCATCAGACGAGGTCTCCCCCCGCCCATCTAAAACAG
   
Sequence Name 28-K020068-022-160-G05C-oriL
GenBank Accession LN498652 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K020068-022-160-G05C-oriL
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGTCTTCTGTTTTA
GATGGTGCTTGGGGGAGACCTCGTCTGATGACGTATGAGCTGCTTCTTTCACTATTCCCG
CCAAAAGTTTCTAGGATATTTAGGTTTTCAACGTCCAGCCCAACAAGATCCACAACATCT
ATAGGATCTTCATTGTAGCCAATTAATTTAGCTTCATTTCGGATGCCATTCTTCAGAACA
TCCTCCAACGGATGGGAGGTTGTTATGTTCTCATGAGCCTGCGGATGAATTACACAAAGA
AGTTCTTGAACAACGAGTTACATATATGAACACTAGATAGCTTAAGATATATATACATCA
CATCAATTGAAGAAATTTGTTACACTACAGTTTCTTTTGATTTTGAGAAAGTACCTTCAG
GTTTGTAATCATCAGCTGTGCAGCCTTTTTTGTGCCCTCTCTTTTGTTCCGACCAGTTGC
AGAAACTGTAAGGCAACAACCATTCTTGGCCTTTTAGTTCACAGTAAAAGCACCATCCTT
TTTCGTCGCTGATATTTCCCGATCCCACTTGTAAGACTGGCAAAGTT
Primers used for PCR AK10 - 8474, show primer details
   
Sequence Name 26-K020068-022-160-G05C-8409
GenBank Accession LN497720 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K020068-022-160-G05C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAAGCCATGTATATTCTTGTGTATTTTA
TCAGGAGATTGTAAATTTAGTGCAATCTTGAATTATCGATTCTATTACTCCACAGGTGGA
CCAAGCAGCAAAACCGCAAAATCCCTCTTGCACGAAACATGTGTTGCTAACTGTTGGAAG
CCACCACACTTCGAATGTTGTGAAGAGGAAGGACCAGGCCACCTGAAATCGTAAGTAACT
TGATCAAACACACTGCTAAATCTCTGAACTTGAGCGCGCTAGAAACTTAATAAACAAAAT
ATCGGTAATTGGAATTTAATGTCATCAATTGTGTGCATGAATACAGATTCGTCTACAAGG
TAATCCTGGAAGTTGAAGATGCGCCCAATATGACATTGGAATGTTATGGTGAGGCTAGAG
CAACGAAGAAAGGTGCAGCAGAGCACGCTGCCCAAGCTGCTATATGGTGCCTCAAGCATT
CTGGATTCCTTTGCTGATGATCTCGAGCGGCTATATATCGGTTAGAAATAGATGTTATCA
TAGGCATATATAACTTTTACCAGAGCTACATATTAGCATATATGATCGACACCTACAATT
TC
   
Sequence Name 25-K020068-022-160-G05C-AK10
GenBank Accession LN497244 [GenBank]
Sequencing primer gene specific primer, AK10: 5'-CAGAAGAGCAATCGAAGAGAACTT-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K020068-022-160-G05C-AK10
TGCCGAAAGCTCTCCGCTCCAAAATCTATGGTTTAAGGTTAGTTTTCAGACATCCTTTTT
TCTCTTTCGAGAAATTGTAGGTGTCGATCATATATGCTAATATGTAGCTCTGGTAAAAGT
TATATATGCCTATGATAACATCTATTTCTAACCGATATATAGCCGCTCGAGATCATCAGC
AAAGGAATCCAGAATGCTTGAGGCACCATATAGCAGCTTGGGCAGCGTGCTCTGCTGCAC
CTTTCTTCGTTGCTCTAGCCTCACCATAACATTCCAATGTCATATTGGGCGCATCTTCAA
CTTCCAGGATTACCTTGTAGACGAATCTGTATTCATGCACACAATTGATGACATTAAATT
CCAATTACCGATATTTTGTTTATTAAGTTTCTAGCGCGCTCAAGTTCAGAGATTTAGCAG
TGTGTTTGATCAAGTTACTTACGATTTCAGGTGGCCTGGTCCTTCCTCTTCACAACATTC
GAAGTGTGGTGGCTTCCAACAGTTACAACCATGTTTCGTGCAAGAGGGATTTTGCGGTTT
TG

Last Updated on 06.12.2018 16:11