Sequences from confirmation amplicon


Confirmation sequences for line 161E06 and hit At2g19800 are shown.
Line ID 161E06
Locus At2g19800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8532881
Primers used for PCR MP33 - 8474, show primer details
   
Sequence Name 41-K023014-022-161-E06C-8409
GenBank Accession LN503927 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K023014-022-161-E06C-8409
GTAGATTTCCCGGACATGAAGCCATTTACATGTAGACCTATGACTTTGTAAGCAAAACAA
ACATACTTTCACTATGGTCTAGTTAATTAAATTATAAGTAAATAATGTTTATTGAAAATC
GATCTTCAAACAATTGGACAGGTGAAGAAGATGAGGAAAGAGTATGGAAAACTTAACAAG
ATGGAAATGAGTATATGGGAATGTTGTGAGTTATTGAACAATGTTGTTGATGAAAGCGAT
CCGGATCTTGATGAGCCTCAAATTCAACACCTTCTCCAAACCGCTGAAGCCATTCGAAGG
GACTATCCCGACGAAGATTGGCTCCATCTCACTGCCCTAATCCATGGTACATGTCTTATT
CTGCTATATCATAAAAAATATACACCACTTTTGAAAAATCTACCATTTATAGTGTTCAAC
AAAGCATCCTGACTATGTATTCTTTAGTCTTTTTTTCTTCTTATGGTTTATATTGAGAAA
TTTCAAAATGATAAGAGCAAACGATTTGTTATGTTGAACAATTTTGAAAGTTCATATAGC
TTCTTATACTGGTTATGTTACTATTCAACATATTAAATATCTTAATGCATACATGTAAAC
TAATCAACATATCTNAGCTACCTATTCTT
   
Sequence Name 42-K023014-022-161-E06C-MP33
GenBank Accession LN504315 [GenBank]
Sequencing primer gene specific primer, MP33: 5'-AACGCAAAGCATTAAAAGAATAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K023014-022-161-E06C-MP33
GATATGTTGATTAGTTTACATGTATGCATTAAGATATTTAATATGTTGAATAGTAACATA
ACCAGTATAAGAAGCTATATGAACTTTCAAAATTGTTCAACATAACAAATCGTTTGCTCT
TATCATTTTGAAATTTCTCAATATAAACCATAAGAAGAAAAAAAGACTAAAGAATACATA
GTCAGGATGCTTTGTTGAACACTATAAATGGTAGATTTTTCAAAAGTGGTGTATATTTTT
TATGATATAGCAGAATAAGACATGTACCATGGATTAGGGCAGTGAGATGGAGCCAATCTT
CGTCGGGATAGTCCCTTCGAATGGCTTCAGCGGTTTGGAGAAGGTGTTGAATTTGAGGCT
CATCAAGATCCGGATCGCTTTCATCAACAACATTGTTCAATAACTCACAACATTCCCATA
TACTCATTTCCATCTTGTTAAGTTTTCCATACTCTTTCCTCATCTTCTTCACCTGTCCAA
TTGTTTGAAGATCGATTTTCAATAAACATTATTTACTTATAATTTAATTAACTAGACCAT
AGTGAAAGTATGTTTGTTTTGCTTACAAAGTCATAGGTCTACATGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGGTTGTATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAAT
GAAAGTACATTTTGATAAAACGACAAATTACGAT

Last Updated on 06.12.2018 16:11