Sequences from confirmation amplicon


Confirmation sequences for line 161G09 and hit At3g60290 are shown.
Line ID 161G09
Locus At3g60290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22282942
Primers used for PCR MG27 - 8474, show primer details
   
Sequence Name 56-K031690-022-161-G09C-8409
GenBank Accession LN509005 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K031690-022-161-G09C-8409
CATTTACAATTGAATATATCCTGAAAGAAAGAAGTACACATGTCTGTTAGGTAAGACACA
TGGCAAGTTCTGCGACTCAGATTCATGCATTGTTACAATCCAAACAAAGAGAAGTTTGAT
GCTTAACTGTCCTATTCATGTGTCTATATTTCTCAGATCATCAAACTCAGCCATGAGTTT
CATTTCTCAGGTCACTAGGACTAGTATGGAATGTACTTATCACATCTACCAATCATGCTT
GATATATGACTTATGAGAAATGCAGTTTTTATCTTTTGCATTTCTATCATACCCAACAAA
GTCCCTTAT
   
Sequence Name 08-K031690-022-161-G09C-MG27
GenBank Accession LN489617 [GenBank]
Sequencing primer gene specific primer, MG27: 5'-GATAAGGGACTTTGTTGGGTATGA-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K031690-022-161-G09C-MG27
GTCATATATCAAGCATGATTGGTAGATGTGATAAGTACATTCCATACTAGTCCTAGTGAC
CTGAGAAATGAAACTCATGGCTGAGTTTGATGATCTGAGAAATATAGACACATGAATAGG
ACAGTTAAGCATCAAACTTCTCTTTGTTTGGATTGTAACAATGCATGAATCTGAGTCGCA
GAACTTGCCATGTGTCTTACCTAACAGACATGTGTACTCTTTCTTTCAGGATATATTCAA
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
AGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATT
TATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTA
CATTCA

Last Updated on 06.12.2018 16:11