Sequences from confirmation amplicon


Confirmation sequences for line 163E03 and hit At5g60830 are shown.
Line ID 163E03
Locus At5g60830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24473067
Primers used for PCR Z70a - 8474, show primer details
   
Sequence Name 51-K014836-022-163-E03C-8409
GenBank Accession LN507335 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K014836-022-163-E03C-8409
GACATGAAGCCATTTACAAGAGATCTACAATTGATAGATCCATATTTTCCGGCAGATGTG
CTATTACCCATGATAAATGCAGAGAGCAACATCAATGACCGCAATGTGAATTATCTAAGA
GGAGAACCATCATACCGTCCCACCAACAGTCCCTTTGGTAAGTAAAAAAAAAAACTTTCA
TCTTCCACTCTTTTAGTTCTTGGGGGGTTAACTTAAGCCAGTATTACTATGATTGATGCC
ATGTTTGCAACTATTGACTACTACAGTAGCAATTACTTACTTGCATTTGTTTGTAGATAC
TAAATAACAACAGTGTTAACACCGAAATTGGGTTTCACATTATTGGTCTAAAGAAACAAA
TTAACTGAAGTAAAGGGTTCCATTCAAACATTGTTAAAACCAGGCNCGTATAGGTTGGAA
ACGAGTCAAAACCAGCTGGTTTATACAATTAGTATAGGCATGTATATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAATAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAAATGTCCGCACCGTTATT
ATAAAATGAAAGTACATTTTGATAAAACGACAAATTAC
   
Sequence Name 53-K014836-022-163-E03C-Z70a
GenBank Accession LN507977 [GenBank]
Sequencing primer gene specific primer, Z70a: 5'-GGTGTTAACAATGTTTGAATGGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K014836-022-163-E03C-Z70a
CCCTTTACTTCAGTTAATTTGCTTCTTTAGACCAATAATGTGATACACAATCTCGGTGTT
AACATTGTTGTTATCTAGTATCTACATACAAATGCAAGTAAGTAATTGCTACTGTAGTAG
TCACTAGTCGCATACATGGCATCAATCATAGTACTACTGGCTTAAGTTAACACACCAAGA
ACTAGAAGAGTGGAAGATGAAAGTTTTTTTTTTTACTTACCAAAGGGACTGTTGGTGGGA
CGGTTTGATGGTTCTCCTCTTAAATAATTCACATTGCGGTCATTGATGTTGCTCTCTGCA
TTTCTCATGGGTAATAGCACATCTGCCGGAAAATATGGATCTTCAATTGTAAATCTCTTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAAATGTCCGCAGC
GTTATTATAAAATGAAAGTCCATTTTGATAAAACGACAAATTACGATCCGTCGTATTTAT
AGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACAT
TCACGTCCAAATGGGGGCTTAAATGAAAAACTTCACGATCGATGCCTTGATTTC

Last Updated on 06.12.2018 16:11