Sequences from confirmation amplicon


Confirmation sequences for line 163H03 and hit At4g23630 are shown.
Line ID 163H03
Locus At4g23630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12319019
Primers used for PCR 09fp - 8474, show primer details
   
Sequence Name 52-K111394-0022-163-H03C-09fp
GenBank Accession LN507854 [GenBank]
Sequencing primer gene specific primer, 09fp: 5'-AATGGGTCGACTTTGTCTTCATAC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K111394-0022-163-H03C-09fp
TTGCAGCAAGATAAAATTTAGTCATCAGGGTTCAGTGATACAAGAGAAGGAAAACACTAA
ATGGTATAGAAGTATAAGCAAAGCATTACCTATGTATGCCAATGTCAAGAAGTTGAAGCA
ACCGCCCAAGATTGAGAGAACCCATAACCCGGCAATAGCCTATTCAATCCAATGGTACAT
TGTTGTCAGATATAAGGATAAACATATTAAGTACCAAGTCCCAAATAGTTTCTACTTAAG
CACATTTGAACTTACAATGAGGAATTTCTTGAGATCCCTTCCTGATGCAATTTCACGGAG
AGAAGAGAATCCACGGTTGATCTCGATTCTGAGCCCAGACGCAAGCTGAAGAATAGGTTC
TTCAGGGATATGAACTTCAGGAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGNTTATT
   
Sequence Name 36-K111393-0022-163-H03C-8409
GenBank Accession LN502269 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K111393-0022-163-H03C-8409
TCCTGAAGTTCATATCCCTGAAGAACCTATTCTTCAGCTTGCGTCTGGGCTCAGAATCGA
GATCAACCGTGGATTCTCTTCTCTCCGTGAAATTGCATCAGGAAGGGATCTCAAGAAATT
CCTCATTGTAAGTTCAAATGTGCTTAAGTAGAAACTATTTGGGACTTGGTACTTAATATG
TTTATCCTTATATCTGACAACAATGTACCATTGGATTGAATAGGCTATTGCCGGGTTATG
GGTTCTCTCAATCTTGGGCGGTTGCTTCAACTTCTTGACATTGGCATACATAGGTAATGC
TTTGCTTATACTTCTATACCATTTAGTGTTTTCCTTCTCTTGTATCACTGAACCCTGATG
ACTAAATTTTTATCTTGCTGCAACAGCTCTTGTGTTGCTCTTCACGGTTCCTCTTGCCTA
CGACAAGTATGAAGACAAATCGGACCCAT

Last Updated on 06.12.2018 16:11