Sequences from confirmation amplicon


Confirmation sequences for line 163H09 and hit At2g17220 are shown.
Line ID 163H09
Locus At2g17220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7489452
Primers used for PCR DE06 - 8474, show primer details
   
Sequence Name 35-K019240-022-163-H09C-8409
GenBank Accession LN501640 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K019240-022-163-H09C-8409
TTCCCGGACATGAAGCCATTTACAATTGAATATATTTCCATGTAGATGCATAATGCTGTT
TGTGATCTGTTATGTGACTCTCTGTTTTGGGCTTGACTTTGTTTTCTTGTGGTTGTAATT
GAAGTAGGGACTTTTAAGTCATCAAACAACACGACGACTACAGGAACATCAAGGGGAAGT
AACATATCTAGCAATAGTGGGTTCTCTGTGGCAAGTGGTGAAGATGCTTACCCCGATGGT
CAGATACTTCCCATTCCGAATTTGAGGATATTCAGCTTAGCAGAACTTAGAGCTTCAACA
AGGAATTTCAGGTCTGAGAATGTTCTTGGAGAGGGAGGATTTGGGAAAGTCTTTAAAGGC
TGGCTTGAAGACAAGACTCCAGGGAAACAAAGCAACGGAACTGTGATTGCTGTTAAGAAA
CT
   
Sequence Name 36-K019240-022-163-H09C-DE06
GenBank Accession LN502040 [GenBank]
Sequencing primer gene specific primer, DE06: 5'-TTCTGCATTCAGTTTCTTAACAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K019240-022-163-H09C-DE06
GTTCCGTTGCTTTGTTTCCCTGGAGTCTTGTCTTCAAGCCAGCCTTTAAAGACTTTCCCA
AATCCTCCCTCTCCAAGAACATTCTCAGACCTGAAATTCCTTGTTGAAGCTCTAAGTTCT
GCTAAGCTGAATATCCTCAAATTCGGAATGGGAAGTATCTGACCATCGGGGTAAGCATCT
TCACCACTTGCCACAGAGAACCCACTATTGCTAGATATGTTACTTCCCCTTGATGTTCCT
GTAGTCGTCGTGTTGTTTGATGACTTAAAAGTCCCTACTTCAATTACAACCACAAGAAAA
CAAAGTCAAGCCCAAAACAGAGAGTCACATAACAGATCACAAACAGCATTATGCATCTAC
ATGGAAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAAT
TACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTT
TATTTCGACGTGTCTACAT

Last Updated on 06.12.2018 16:11