Sequences from confirmation amplicon


Confirmation sequences for line 164H12 and hit At4g31840 are shown.
Line ID 164H12
Locus At4g31840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15402176
Primers used for PCR NA13 - 8474, show primer details
   
Sequence Name 13-K101562-0022-164-H12C-8409
GenBank Accession LN491974 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K101562-0022-164-H12C-8409
ATTTACATTGAATAAATGGAACACAAAGGTGAAGCTTGACCAAGCTGGTCCGGTCTACTT
CGTCAGTGGAACAGAGGGTCATTGTCAAAAGGGTCAGAAGCTACGCCTAGTCGTCATCAC
TCCTCGTAACTCTGCTTTCTCTCCAGGTCCTTCGCCATCTGAGTTCGACGGTCCAGCCGT
AGCTCCTACTAGTGGTGCCGCTAAGCTCGCCGGCGGATTTAGTGTCGTATTTGGGCTTGT
TCTTGGATTATGGGCCTTCTTCTTTTAAAAGACAATTAGTCTTAATTCTATTTATTAATT
TTGGAATATAAAGTAGAAATCTTTGTTATTTACTACATTAAATTTATTTTAATCTAAATT
GTTCTTCCTCTTTTTCAATTCTGGATAAATTAGTTGGATCTATCAACAATCAACAAAGAA
AACAAAACAAAAGTGAATGAGAATTTTGAATTTAGTACCCAAAAAGGTAATTTACAGCAA
CACAAAGAAACCTGGACTGTCGTGAAAAGAGGCCTAAAAATGGGCCTGAATTGGGCCTAA
GAATATTTTG
   
Sequence Name 13-K101571-0022-164-H12C-NA13
GenBank Accession LN491975 [GenBank]
Sequencing primer gene specific primer, NA13: 5'-CAAAATATCTTTAGGCCCAATTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K101571-0022-164-H12C-NA13
CGTCCGGTTTCTTTGTGTTGCTGTAAATTACCTTTTTGGGTACTAAATTCAAAATTCTCA
TTCACTTTTGTTTTGTTTTCTTTGTTGATTGTTGATAGATCCAACTAATTTATCCAGAAT
TGAAAAAGAGGAAGAACAATTTAGATTAAAATAAATTTAATGTAGTAAATAACAAAGATT
TCTACTTTATATTCCAAAATTAATAAATAGAATTAAGACTAATTGTCTTTTAAAAGAAGA
AGGCCCATAATCCAAGAACAAGCCCAAATACGACACTAAATCCGCCGGCGAGCTTAGCGG
CACCACTAGTAGGAGCTACGGCTGGACCGTCGAACTCAGATGGCGAAGGACCTGGAGAGA
AAGCAGAGTTACGAGGAGTGATGACGACTAGGCGTAGCTTCTGACCCTTTTGACAATGAC
CCTCTGTTCCACTGACGAAGTAGACCGGACCAGCTTGGTCAAGCTTCACCTTTGTGTTCC
ATTTATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGC

Last Updated on 06.12.2018 16:11