Sequences from confirmation amplicon


Confirmation sequences for line 165C04 and hit At1g09930 are shown.
Line ID 165C04
Locus At1g09930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3228079
Primers used for PCR RK18 - 8474, show primer details
   
Sequence Name 21-K015891-022-165-C04C-8409
GenBank Accession LN495447 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K015891-022-165-C04C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCAGTGGCATGGTACTTGCTG
ACATCAGTAGAAAACATCTGCCAGAAGGAGTTGCTTCCTCCAAACAGTCCATGGACATGT
CCAAGTGACAGAGTTTTCTTCGACGCATCAGTGATTTGGGGATTAGTAGGACCCAAGAGA
ATCTTTGGTCGGCTAGGAAACTACCCTGCACTAAACTGGTTCTTCTTAGGTGGACTAATA
GGACCGGTCCTCGTGTGGCTTCTCCAAAAAGCCTTCCCAACGAAAACATGGATCTCGCAG
ATCAATCTCCCAGTTCTTTTAGGAGCAACAGCAGCAATGCCGCCAGCGACAAGCGTGAAC
TTCAACTGCTGGATCATAGTAGGAGTGATATTCAATTACTTTGTGTTCAAGTACTGCAAA
AAGTGGTGGCAGAGGTATAACTACGTGCTATCGGCAGCTTTGGATGCGGGATTGGCGTTC
ATGGGAGTGTTGTTATACTTTAGTTTGACGATGAATG
   
Sequence Name 22-K015891-022-165-C04C-RK18
GenBank Accession LN495889 [GenBank]
Sequencing primer gene specific primer, RK18: 5'-TATCGATATTCCATTCATCGTCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K015891-022-165-C04C-rk18
AAGTATAACAACACTCCCATGAACGCCAATCCCGCATCCAAAGCTGCCGATAGCACGTAG
TTATACCTCTGCCACCACTTTTTGCAGTACTTGAACACAAAGTAATTGAATATCACTCCT
ACTATGATCCAGCAGTTGAAGTTCACGCTTGTCGCTGGCGGCATTGCTGCTGTTGCTCCT
AAAAGAACTGGGAGATTGATCTGCGAGATCCATGTTTTCGTTGGGAAGGCTTTTTGGAGA
AGCCACACGAGGACCGGTCCTATTAGTCCACCTAAGAAGAACCAGTTTAGTGCAGGGTAG
TTTCCTAGCCGACCAAAGATTCTCTTGGGTCCTACTAATCCCCAAATCACTGATGCGTCG
AAGAAAACTCTGTCACTTGGACATGTCCATGGACTGTTTGGAGGAAGCAACTCCTTCTGG
CAGATGTTTTCTACTGATGTCAGCAAGTACCATGCCACTGATATATTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAA

Last Updated on 06.12.2018 16:11