Sequences from confirmation amplicon


Confirmation sequences for line 165C10 and hit At4g33510 are shown.
Line ID 165C10
Locus At4g33510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16118425
Primers used for PCR 03fm - 8474, show primer details
   
Sequence Name 67-K111632-0022-165-C10C-03fm
GenBank Accession LN511842 [GenBank]
Sequencing primer gene specific primer, 03fm: 5'-CTTTGATGCAGGTGAGTGATAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K111632-0022-165-C10C-03fm
CGTTTAAAGATGAGATCTAACCCTCAGAACAGCCTGGAAGGATTACGGTTATAGTGAGAA
TGGGAGCTGAGAATATGCGGGTCAAGCTTCCTAATTTGATCAGAGCAGTCCGTGGAGCCG
GTCAGATTGTGACTTGGGTTAGTGATCCAATGCACGGAAACACAATCATGGCTCCTGGTG
GGCTAAAAACTCGTTCTTTCGATGCAATCAGGGTAATCATTCGAACTTACTTCTTCATCC
AACTCTATGCATAATCTTGCTTATGATTCATGTAGTTACCCATTAAATTATATGGTGTAT
CAGGCGGAATTGAGAGCGTTCTTCGACGTCCATGATCAAGAAGGGAGTTTCCCTGGCGGG
GTTCATTTAGAAATGACTGGTCAAAACGTGACAGAATGTGTCGGAGGGTCACGCACCATC
ACTTACAACGATCTAAGCTCACGCTATCACTGCAATGTGACAGGATATATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTT
ATT
   
Sequence Name 51-K111631-0022-165-C10C-8409
GenBank Accession LN507543 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K111631-0022-165-C10C-8409
TTTCATTGAATATATCCTGTCACATTGCAGTGATAGCGTGAGCTTAGATCGTTGTAAGTG
ATGGTGCGTGACCCTCCGACACATTCTGTCACGTTTTGACCAGTCATTTCTAAATGAACC
CCGCCAGGGAAACTCCCTTCTTGATCATGGACGTCGAAGAACGCTCTCAATTCCGCCTGA
TACACCATATAATTTAATGGGTAACTACATGAATCATAAGCAAGATTATGCATAGAGTTG
GATGAAGAAGTAAGTTCGAATGATTACCCTGATTGCATCGAAAGAACGAGTTTTTAGCCC
ACCAGGAGCCATGATTGTGTTTCCGTGCATTGGATCACTAACCCAAGTCACAATCTGACC
GGCTCCACGGACTGCTCTGATCAAATTAGGAAGCTTGACCCGCATATTCTCAGCTCCCAT
TCTCACTATAACCGTAATCCTTCCAGGCTTGTTCTGAGGGTTTAGTATCTCTATCAGCTT
AACCAGTTCACTAGGGACCATTTTATCACTCACCTGCATCAAA

Last Updated on 06.12.2018 16:11