Sequences from confirmation amplicon


Confirmation sequences for line 165G02 and hit At1g02830 are shown.
Line ID 165G02
Locus At1g02830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:625312
Primers used for PCR 13xm - 8474, show primer details
   
Sequence Name 51-K119512-0022-165-G02C-13xm
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 13xm: 5'-AAGAGAATAGCTCGTAGCTCAGCC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K119512-0022-165-G02C-13xm
TAACCGGTTTAGGATCTGGAACCCATGGTGCCTTCTCGCTTGAAGCTTCCCCAGCTCTCT
TCTTCATCTCCGCGGTCGAAACGCTTCCATCTAACGCCGTTTTGGCTGCGGCGGCAAACC
CTCGTCTGAAGAGAATCCAAAATCCAAAACGTCAGCGTTTTGTTAAAGCGACGAGAAAAC
AGAGCAATACATAACGTTTTTGTTTCAGAAACAGATGTTACCTGAAAACGGCGTTCGAGA
GCTTTTCGGAGCCGAAGACGGATTGGATCTTAGCGTTAGCGAGAGAACGAGCCATTGTGG
TTTATAGGTTTTACGAGTTTTGGAGAAGAAGAAAAACAGCGCAAAAGAGAAGTTTCTCAG
GTTCTTGATCTGATGACTGCTGTTACCTTTTTGCGGCAACGAGATCGAACAAACTTGGTC
GTCCTATCTCTCTTGACAGATTTCAAAATGCTTTGCAGGATAGATGAGAAGGAAGAGAGA
TGAGAGGAGGGTTTATATAGAGGAAGCAGAGCAGATAGGCTGCGTCAATTTAGGCTTTCA
CCCAAAGTTATTGTTTTCTGTTTCTTTTGATTTTTTTTTTTGGGTTTAGGGTTTTAAAGT
TAGTTGTAACTTATAATTAAAATTGTTTTCAAAGTCGTTTTGTATGTAATTAACAACTTG
TTTTACTTATCTGGATTTAAAATTAATTAACTCATCTAATATACAAGTATACTATAGTTT
GACCAAAACCAATTTTTATCTTCTTTTTTTT
   
Sequence Name 19-K119511-0022-165-G02C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K119511-0022-165-G02C-8409
CAAATCTGGTTTTGAATTCGGTACTTGAAGTATCTGACAAAGAAATATTTGAAGAAGTAC
AATCTCCGTGATTGGCTACGAGTGATTGCATCTAACAAAGACAAAAATGTCTACGAGGTA
CGCTACTTCAGGATTGACGATGAAGTAGCTAGTTACGAGGAAGATTAAGCTCGACCTTCT
TCATTCATTTTTAATTACACTTGGTCTATATTTTTCCTGCTTAGGACGGATCTTTATTAA
TTGTCCAAGTTTTTGCTGTCGTATGATTTTGGTGGTTAAAATTGACACAAGGATGAGGAC
TAAGTTCATTTCAAAATTCTGAAAGAAAGAGTGACTAAATGGCCGACTCTTTTTGAGGCC
GTGTTGGTTCGGCAGAATTGAGTGGTTCCGACTCAATTTAGTTTAGGAGATCAAAAAATA
TCTAAATATATGCAATCACAATTATTTAGGAGATAAAAAAATATCTAAATATATCATTAA
TTTCTTTTGAGCATTATGAATACGTACATATGGTAATTTCATATATGAACATTTATATTT
TCGGTTGACTACAAAAAAAAGAAGATAAAAATTGGTTTTGGTCAAACTATAGTATACTTG
TATATTAGATGAGTTAATTAATTTTAAATCCAGATAAGTAAAACAAGTTGTTAATTACAT
ACAAAACGACTTTGAAAACAATTTTAATTATAAGTTACAACTAACTTTAAAACCCTAAAC
CCAAAAAAAAAATTCAAAAGAAACGGAAAACAATAACTTTGGGTGAAAGCCTAAATTGAC
GCAGCCTATCTGCTCTGCTTCCTCTATATAAACCCTCCTCTCATCTCTCTTCCTTCTCAT
CTATCCGGCAAAGCATTTTTG

Last Updated on 06.12.2018 16:11