Sequences from confirmation amplicon


Confirmation sequences for line 166G09 and hit At4g38430 are shown.
Line ID 166G09
Locus At4g38430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:17987895
Primers used for PCR MF25 - 8474, show primer details
   
Sequence Name 17-K031692-022-166-G09C-MF25
GenBank Accession LN493737 [GenBank]
Sequencing primer gene specific primer, MF25: 5'-TACCACATTCGTCTTCGTAGTCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K031692-022-166-G09C-MF25
ACCATTGATTACATTCTTCTATTTTCATCTTAATCTCTTTCCGGCGTCACTCCCGTTACT
CTTCTTGAACTCAGATTCCCTGCATACGACCATGCTTTCTCTGTTTCTCCCACTATTCCC
TTCTCTCTCGTCCCATTCCTCTTCACTGAATACAGCGGCGGTGCTCTCCTCGGGCTTCTT
GATGCTACACTTAGGGCAGGCGTTGCAAATGGTGAACCGTGCGGAGAGCTCTGCACAGAG
AACGCTTTTTGAGGGTTGAGTCCATTGATGCTGAATAGGGATAGCGATTCTGTGACAGAG
ATGGATCTTCTCATGGCATCATCAACATAAAGCACATCATCTATTCTGGCCGTGATGTTG
AAGGCCATGCTCTCCATCACCCTAGAGTAACTCTCCAGAATTGATTGCCCTACATCCTGC
AAGAACACACACAAACTCTTAAATTATCTAAGCATCGTTTCATGATGCAACCAGCAAAGC
ACACTCCAAACTATAATGTACCTTGTTGTACTGAATCTTGTTCATGTCCAGCGTTGTCTG
GGGAAGTCCCGGGAAACGGATCCTGAGACTCTGCAAGAGGGTCTCGGCTCTCTGCACAAG
GAAATCATTTCTTTCGTTGTCATTAACAAGGCCTTTAACCTTGCCACCCCAAGAAGACAG
CTTTAGTTTCGCCTGCTTCTTGTGCCTCCTCCCATTCTTTTGTCTCCAAACATGCACAGC
CGCCTCAATCCGGTTGGCGATNTCAAGAGTCTGATGTTCCGATGAGAGATCAAGACAGTC
CAGGAGGCATTCGGCAAAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCTATTTGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTTCATTCAAAAA
   
Sequence Name 65-K031692-022-166-G09C-8409
GenBank Accession LN511321 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K031692-022-166-G09C-8409
TTACAATTGAATATATTTTGCCGAATGCCTCCTGGACTGTCTTGATCTCTCATCGGAACA
TCAGACTCTTGAAATCGCCAACCGGATTGAGGCGGCTGTGCATGTTTGGAGACAAAAGAA
TGGGAGGAGGCACAAGAAGCAGGCGAAACTAAAGCTGTCTTCTTGGGGTGGCAAGGTTAA
AGGCCTTGTTAATGACAACGAAAGAAATGATTTCCTTGTGCAGAGAGCCGAGACCCTCTT
GCAGAGTCTCAGGATCCGTTTCCCGGGACTTCCCCAGACAACGCTGGACATGAACAAGAT
TCAGTACAACAAGGTACATTATAGTTTGGAGTGTGCTTTGCTGGTTGCATCATGAAACGA
TGCTTAGATAATTTAAGAGTTTGTGTGTGTTCTTGCAGGATGTAGGGCAATCAATTCTGG
AGAGTTACTCTAGGGTGATGGAGAGCATGGCCTTCAACATCACGGCGAGAATACATGATG
TGCTTTATGTTGATGATGCCATGAGAAGATCCATCTCTGTCACAGAATCGCTAGCCCTAT
TCAGGATCAATGGAGACAACCCTCAAAAAGCATTCTCTATGCAGAGCTCTCCTCACGGAT
AACCATTTGCATCACCTGCCCTAAGTGTAACATCGGGAAACCCGAGGAGAGCACCACAAC
TGTATTCAA

Last Updated on 06.12.2018 16:11