Sequences from confirmation amplicon


Confirmation sequences for line 167A04 and hit At5g13270 are shown.
Line ID 167A04
Locus At5g13270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4248944
Primers used for PCR AP11 - 8474, show primer details
   
Sequence Name 68-K106402-0022-167-A04C-8409
GenBank Accession LN512025 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K106402-0022-167-A04C-8409
GCCTTTACAATTGAATATATCCTGCCGAGAATTGAGGCTTGCAGAGAATTGAGGTCTTTA
TCTCATGGGAGACTCTTGCACGATCGCATGCGGATGGGTATCGAGAATCCTTCTGTGCTT
CTTCAGAACTGTGTGCTTCAAATGTACTGTGAGTGTCGGAGCTTGGAAGATGCAGATAAG
CTGTTCGATGAAATGTCTGAGTTAAATGCAGTTTCTCGTACAACGATGATATCTGCTTAC
GCCGAACAAGGAATTTTGGATAAAGCTGTTGGTTTGTTCTCGGGAATGCTAGCGTCAGGC
GACAAGCCTCCTTCTTCCATGTATACGACACTCTTGAAATCTTTGGTAAACCCCAGGGCT
TTAGACTTTGGTAGACAGATTCATGCTCATGTTATACGTGCTGGGTTATGTAGCAACACA
TCTATAGAGACCGGGATCGTGAACATGTACGTGAAATGTGGTTGGTTAGTGGGTGCTAAG
CGAGTGTTCGATCAGATGGCAGTGAAGAAACCAGTGGCTTGTACGGGATTGATGGTAGGT
TATACTCAAGCTGGAAGAGCAAGAGATGCTTTGAAACTATTTGTGGATTTGGTAACGGAA
GGTGTTGAATGGGACAGTTTTGTGTTTTCAGTTGTTCTTAAAGCGT
   
Sequence Name 84-K106403-0022-167-A04C-AP11
GenBank Accession LN514434 [GenBank]
Sequencing primer gene specific primer, AP11: 5'-AAGAGAAGCACACGCTTTAAGAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K106403-0022-167-A04C-AP11
CATTCACACCTTCCGTTACCAAATCCACAAATAGTTTCAAGCATCTCTTGCTCTTCCAGC
TTGAGTATAACCTACCATCAATCCCGTACAAGCCACTGGTTTCTTCACTGCCATCTGATC
GAACACTCGCTTAGCACCCACTAACCAACCACATTTCACGTACATGTTCACGATCCCGGT
CTCTATAGATGTGTTGCTACATAACCCAGCACGTATAACATGAGCATGAATCTGTCTACC
AAAGTCTAAAGCCCTGGGGTTTACCAAAGATTTCAAGAGTGTCGTATACATGGAAGAAGG
AGGCTTGTCGCCTGACGCTAGCATTCCCGAGAACAAACCAACAGCTTTATCCAAAATTCC
TTGTTCGGCGTAAGCAGATATCATCGTTGTACGAGAAACTGCATTTAACTCAGACATTTC
ATCGAACAGCTTATCTGCATCTTCCAAGCTCCGACACTCACAGTACATTTGAAGCACACA
GTTCTGAAGAAGCACAGAAGGATTCTCGATACCCATCCGCATGCGATCGTGCAAGAGTCT
CCCATGAGATAAAGACCTCAATTCTCTGCAAGCCTCAATTCTCGGCAGGATATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC

Last Updated on 06.12.2018 16:11