Sequences from confirmation amplicon


Confirmation sequences for line 167A10 and hit At5g04900 are shown.
Line ID 167A10
Locus At5g04900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1435434
Primers used for PCR MG30 - 8474, show primer details
   
Sequence Name 09-K031691-022-167-A10C-MG30
GenBank Accession LN490059 [GenBank]
Sequencing primer gene specific primer, MG30: 5'-TCACTAAGCTTCCGTTTCTCTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K031691-022-167-A10C-MG30
TCTCGCAACTGTCGTTGTTACGCGCCAAAACCTCACGGTTACTCCGTCGTCTGCGGCGGT
GGAAGCTCGTATCTCAGGGAAAAGAGAACCTATGACGCCTCCGTATAACATATTGATCAC
TGGCTCAACCAAAGGTTTCATTCACTCTGTCTTTGTCAATTGACTCGAGTTTTTAATCTT
CAGAAGGGAAAAAACATATTTTTTTATCTCATTGTGTCTCTAAAGAGTGGTTTTTGATTT
ATTGATTGTTCAGGTATAGGATATGCATTAGCTAGAGAGTTTCTGAAAGCAGGCGACAAT
GTTGTGATTTGCTCTAGATCAGGTTAGAACCTGTCCCCATTGTTGTCTTGAACTCCTTTT
CTAGAGTTTGTGTGTCTGTAAGGAAACATGCAAGCAAGTAATAGAACCAATCTTCAGTAG
AATTTTGCTGTTATGGCTGTTCCTGTCTTGCAGCTGAACGAGTTGAGACTGCTGTACAGG
ATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGAT
CCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTC
GACGTGTCTACATTCAC
   
Sequence Name 57-K031691-022-167-A10C-8409
GenBank Accession LN509318 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K031691-022-167-A10C-8409
CATTTACAATTGAATATATCCTGTACAGCAGTCTCAACTCGTTCAGCTGCAAGACAGGAA
CAGCCATAACAGCAAAATTCTACTGAAGATTGGTTCTATTACTTGCTTGCATGTTTCCTT
ACAGACACACAAACTCTAGAAAAGGAGTTCAAGACAACAATGGGGACAGGTTCTAACCTG
ATCTAGAGCAAATCACAACATTGTCGCCTGCTTTCAGAAACTCTCTAGCTAATGCATATC
CTATACCTGAACAATCAATAAATCAAAAACCACTCTTTAGAGACACAATGAGATAAAAAA
ATATGTTTTTTCCCTTCTGAAGATTAAAAACTCGAGTCAATTGACAAAGACAGAGTGAAT
GAAACCTTTGGTTGAGCCAGTGATCAATATGTTATACGGAGGCGTCATAGGTTCTCTTTT
CCCTGAGATACGAGCTTCCACCGCCGCAGACGACGGAGTAACCGTGAGGTTTTGGCGCGT
AACAACGACAGTTGCGAGACCAAAGCGTTCTGCCAGTAACCGACGCCGGCAAATAGGAGA
GAGAAACGGAAGCTTAGTGA

Last Updated on 06.12.2018 16:11