Sequences from confirmation amplicon


Confirmation sequences for line 167C06 and hit At1g66130 are shown.
Line ID 167C06
Locus At1g66130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24615461
Primers used for PCR 09of - 8474, show primer details
   
Sequence Name 22-K114794-0022-167-C06C-09of
GenBank Accession LN496228 [GenBank]
Sequencing primer gene specific primer, 09of: 5'-TGTATCAGATTCGCATCAAAGTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K114794-0022-167-C06C-09of
TCGCCATCAGCGATCCATCACTCGAAACCGCAAAAACCTTCGCCACCGGGAACAATTTAT
CGCCGGAGACCGTCACGATATACGGAAGCTACGAGGAGCTTTTAAACGATGCAAACGTCG
ACGCCGTTTACTTGACGATGCCGGTGACGCAACGAGCGAGATGGGCTGTGACGGCGGCAG
AGAAGAAGAAGCATGTTCTTGTTGAGAAACCACCAGCTCAAGACGCGACGGAGCTGGAGA
AGATTGTGGAGGCGTGTGAATATAACGGTGTTCAGTTTATGGACGGTACGATTTGGTTGC
ATCATCAACGGACGGTTAAGATGGCTTCAAATGAGTATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 09-K114793-0022-167-C06C-8409
GenBank Accession LN490297 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K114793-0022-167-C06C-8409
ATACTCATTTGAAGCCATCTTAACCGTCCGTTGATGATGCAACCAAATCGTACCGTCCAT
AAACTGAACACCGTTATATTCACACGCCTCCACAATCTTCTCCAGCTCCGTCGCGTCTTG
AGCTGGTGGTTTCTCAACAAGAACATGCTTCTTCTTCTCTGCCGCCGTCACAGCCCATCT
CGCTCGTTGCGTCACCGGCATCGTCAAGTAAACGGCGTCGACGTTTGCATCGTTTAAAAG
CTCCTCGTAGCTTCCGTATATCGTGACGGTCTCCGGCGATAAATTGTTCCCGGTGGCGAA
GGTTTTTGCGGTTTCGAGTGATGGATCGCTGATGGCGATNATGACGGCGTTTGAGGATTC
TGTTACTGTTCTGACGAACTTTGATGCG

Last Updated on 06.12.2018 16:11