Sequences from confirmation amplicon


Confirmation sequences for line 167G11 and hit At5g50000 are shown.
Line ID 167G11
Locus At5g50000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20343646
Primers used for PCR 02zb - 8474, show primer details
   
Sequence Name 25-K123596-0022-167-G11C-02zb
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 02zb: 5'-ATCAAATAGGATGTTCCTGTCGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K123596-0022-167-G11C-02zb
AGACAGTTGTCCATTGACTAGCTATATCAAATATATGATTAGAGATGATGATATACGTGA
CCATAAATATTTAATAAGCAACTCTCACAAATAGAGCAAAACAAGAAATGTTGAAGAATA
TGGAAACCAAATCATACTCTTTACGTAAATTTTAGCAACCATTAGATCCCTAACAAGCCT
TCCCATTTTTTTTCCTTCTGGATTATAAAAAGAAAAAGAAAAGAAGCAAGGACACGAATC
TTGAGTTTTGAATGATCTTAGATTGGTCTTTGGGAATTAAGGACCACGTTTCCTTCGGAA
GCATAGGCAACCCTGTTGCTGGTCGTTAGGAATCATCCCTCCTCCCTTGGTTGTGTCAAT
ACTCTCCAACATTGGTACAACCTCATCCATCTCCGGTCTCTTGTCCGGATTTGCATCCCA
ACATCGCTTCATCACAGCTGCAAGTGCGCTCGGGCAACACCTCGGTATATCGGGTCTCAG
GTTCTGCATTGATCATATGTAGCAGCATATTGCATATAGATTTTTTACTTCAGTGAAATG
ATGAAATGGTTACACTACTCTAGATCACATTTTTTACTTCAGTGATTCCTCAAGATTTAC
AACAAAATATAATTGTGAAACAACCAATATGCACATTTCAATTAAATTAACTTTTCTTTG
CTTAATCTATTCAACCATACCTGGCGGACGACGGCTGAAGTGACCTCAGAGAAAGTAAGG
TCAGGGTATGGCATGTCACAGCAATATATCTCCCATAAGCAGATCCCGAAACTGTATACA
TCGCATTTTCGATTATACGGGTTTCCATTGAGAACCTGCAGCCAAAGTTTATTTGTTATA
AAAAGTTGGTTTGTTACCAATCTTGATGATGCATTATCACTATAGTACCTCAGGCGCCAT
GTAGCCAAGTGTGCCAGTCTCTCCTGTCATGTCATTAGGGTTAGATGCTTCAACTCTCGC
AACTCCAAAATCTGCGATTTTAA
   
Sequence Name 09-K123595-0022-167-G11C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K123595-0022-167-G11C-8409
CATTTACATTGAATATGTAAATACTCATTGTTATTATTTTTGTTATTGTTATGTTGCTTT
TAGGTTGAGTTACCTTCATTCGCAGAAGATTGTTCACCGAGATGTAAAGACAGAGAATAT
GTTGTTAGACAAAACCCGGACTGTTAAAATCGCAGATTTTGGAGTTGCGAGAGTTGAAGC
ATCTAACCCTAATGACATGACAGGAGAGACTGGCACACTTGGCTACATGGCGCCTGAGGT
ACTATAGTGATAATGCATCATCAAGATTGGTAACAAACCAACTTTTTATAACAAATAAAC
TTTGGCTGCAGGTTCTCAATGGAAACCCGTATAATCGAAAATGCGATGTATACAGTTTCG
GGATCTGCTTATGGGAGATATATTGCTGTGACATGCCATACCCTGACCTTACTTTCTCTG
AGGTCACTTCAGCCGTCGTCCGCCAGGTATGGTTGAATAGATTAAGCAAAGAAAAGTTAA
TTTAATTGAAATGTGCATATTGGTTGTTTCACAATTATATTTTGTTGTAAATCTTGAGGA
ATCACTGAAGTAAAAAATGTGATCTAGAGTAGTGTAACCATTTCATCATTTCACTGAAGT
AAAAAATCTATATGCAATATGCTGCTACATATGATCAATGCAGAACCTGAGACCCGATAT
ACCGAGGTGTTGCCCGAGCGCACTTGCAGCTGTGATGAAGCGATGTTGGGATGCAAATCC
GGACAAGAGACCGGAGATGGATGAGGTTGTACCAATGTTGGAGAGTATTGACACAACCAA
GGGAGGAGGGATGATTCCTAACGACCAGCAACAGGGTTGCCTATGCTTCCGAAGGAAACG
TGGTCCTTAATTCCCAAAGACCAATCTAAGATCATTCAAAACTCAAGATTCGTGTCCTTG
CTTCTTTTCTTTTTCTTTTTATAATCCAGAAGGAAAAAAAATGGGAAGGG

Last Updated on 06.12.2018 16:11