Sequences from confirmation amplicon


Confirmation sequences for line 168B02 and hit At5g64210 are shown.
Line ID 168B02
Locus At5g64210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25684873
Primers used for PCR 04gz - 8474, show primer details
   
Sequence Name 49-K112176-0022-168-B02C-04gz
GenBank Accession LN506888 [GenBank]
Sequencing primer gene specific primer, 04gz: 5'-CGTACCATTTGGGTTTGACTAACT-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K112176-0022-168-B02C-04gz
AATGCTTTGATCCAACCGCCGCTATGTTCGAATTTTTCGGATTGATTTTAGGTGAAGAAG
CATTCCTCCAACCATTCCTGGTACTGCAGCTACTGTCTCTAGCATCATCGCTCTGCATCC
ATATCGTCTCTGCTGACCAAACATATATTTAACATTAAGGAACAAAAAATGTAAAACATT
AGCATGTGAAGTGAAACAGTGAATCCATACGTATGTATATATGTGAATCATGGTAGGATA
ATATGTGAAGTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTC
   
Sequence Name 33-K112175-0022-168-B02C-8409
GenBank Accession LN501052 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K112175-0022-168-B02C-8409
TTTACATTGATATATCCTGAACTTCACATATTATCCTACCATGATTCACATATATACATA
CGTATGGATTCACTGTTTCACTTCACATGCTAATGTTTTACATTTTTTGTTCCTTAATGT
TAAATATATGTTTGGTCAGCAGAGACGATATGGATGCAGAGCGATGATGCTAGAGACAGT
AGCTGCAGTACCAGGAATGGTTGGAGGAATGCTTCTTCACCTAAAATCAATCCGAAAATT
CGAACATAGCGGCGGTTGGATCAAAGCATTACTCGAAGAAGCAGAGAACGAGAGGATGCA
TCTAATGACGATGATGGAGTTAGTCAAACCAAANTTGGTACG

Last Updated on 06.12.2018 16:11