Sequences from confirmation amplicon


Confirmation sequences for line 168G01 and hit At4g02420 are shown.
Line ID 168G01
Locus At4g02420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1064908
Primers used for PCR NK33 - 8474, show primer details
   
Sequence Name 07-K102723-0022-168-G01C-8409
GenBank Accession LN489223 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K102723-0022-168-G01C-8409
GAGCATTTACATTGAATATATCATTGAGCTCTGCATCTAACAAGACGTTGCTGGCTTTGA
CGTCGCGGTGAATCACCACTTGTTCCCACTCCTCGTGGAGATAAAATAAGGCAGAGGCCA
CGCCATTAATGACTTTAAACCTCTGTTTCCAATCGAGGGTTACCTCTGGACTATTGTACA
AATACTTGTCTAAACTTCCATTGGGCATGTAATCGTACACCAGAAGAAGCTCGTCTCTCC
GACGGCAATAACCCACGAGAGGAACCAAGTTCCGGTGACTCATCTGTCCAATACTCACGA
TCTCAGCCACGAACTCTTTCAAACCTTGTCTAGATTCGTTCGAGACTCTTTTCACGGCAA
TCTCCTTCTTCGTCTTGGGCATGATACCTTTGTAACCACTCCC
   
Sequence Name 23-K102728-0022-168-G01C-NK33
GenBank Accession LN496509 [GenBank]
Sequencing primer gene specific primer, NK33: 5'-CGGGAGTGTTTACAAAGGTATCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K102728-0022-168-G01C-NK33
GAGTCTCGAACGAATCTAGACAGGTTTGAAAGAGTTCGTGGCTGAGATCGTGAGTATTGG
ACAGATGAGTCACCGGAACTTGGTTCCTCTCGTGGGTTATTGCCGTCGGAGAGACGAGCT
TCTTCTGGTGTACGATTACATGCCCAATGGAAGTTTAGACAAGTATTTGTACAATAGTCC
AGAGGTAACCCTCGATTGGAAACAGAGGTTTAAAGTCATTAATGGCGTGGCCTCTGCCTT
ATTTTATCTCCACGAGGAGTGGGAACAAGTGGTGATTCACCGCGACGTCAAAGCCAGCAA
CGTCTTGTTAGATGCAGAGCTCAATGATATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTAC
ATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAAT
TATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCAC

Last Updated on 06.12.2018 16:11