Sequences from confirmation amplicon


Confirmation sequences for line 169B06 and hit At1g74950 are shown.
Line ID 169B06
Locus At1g74950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28149374
Primers used for PCR 10hw - 8474, show primer details
   
Sequence Name 40-K112667-0022-169-B06C-10hw
GenBank Accession LN503759 [GenBank]
Sequencing primer gene specific primer, 10hw: 5'-TGTGTATGAATTTGTGGAGATGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K112667-0022-169-B06C-10hw
GCCTGGTTAGTCATTAAGTAGATTTTGTTTTGCAGTTGTTTGAGAGATTGGTGAATGATA
ATAATCTTTACTTTTGGATCAATTTTCGCAGGAGGTTCACGTCAGCCTACAATGATGAAT
CTGTTCCCTTGTGAAGCTTCAGGAATGGATTCTTCTGCTGGTCAAGAAGACATTAAACCG
AAGACTATGTTTCCGAGACAATCAAGCTTTTCTTCTTCCTCTTCCTCTGGGACCAAAGAA
GATGTACAGATGATCAAAGAGACTACTAAGTATGTGTTTATATGTATATAGTGAAAAGAA
TCGTCTCATATAGTAAGCTATGTTTCATCAGTTTAACTAATTTTTGTGTGATTTTGAACA
GATCTGTGAAGCCAGAGTCTCAATCTGCTCCGTTGACTATATTCTACGGTGGTCGAGTTA
TGGTGTTTGATGATTTTTCTGCTGAGAAAGCTAAAGAAGTCATTGATTTGGCTAACAAAG
GAAGTGCCAAAAGCTTCACATGTTTCACATGAAGTATGATGCATGCATGTAATCTACATG
TAAATATACATGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 08-K112666-0022-169-B06C-8409
GenBank Accession LN489800 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K112666-0022-169-B06C-8409
CATTTCAATTGAATATATCATGTATATTTACATGTAGATTACATGCATGCATCATACTTC
ATGTGAAACATGTGAAGCTTTTGGCACTTCCTTTGTTAGCCAAATCAATGACTTCTTTAG
CTTTCTCAGCAGAAAAATCATCAAACACCATAACTCGACCACCGTAGAATATAGTCAACG
GAGCAGATTGAGACTCTGGCTTCACAGATCTGTTCAAAATCACACAAAAATTAGTTAAAC
TGATGAAACATAGCTTACTATATGAGACGATTCTTTTCACTATATACATATAAACACATA
CTTAGTAGTCTCTTTGATCATCTGTACATCTTCTTTGGTCCCAGAGGAAGAGGAAGAAGA
AAAGCTTGATTGTCTCGGAAACATAGTCTTCGGTTTAATGTCTTCTTGACCAGCAGAAGA
ATCCATTCCTGAAGCTTCACAAGGGAACAGATTCATCATTGTAGGCTGACGTGAACCTCC
TGCGAAAATTGATCCAAAAGTAAAGATTATTATCATTCACCAATCTCTCAAACAACTGCA
AAACAAAATCTACTTAATGACTAAACCAGGCCTATGGCTTTAGAGGATTATGCTTAAACC
AAACACAGAACCATCTCCACAAATTCATACAC

Last Updated on 06.12.2018 16:11