Sequences from confirmation amplicon


Confirmation sequences for line 169C03 and hit At1g11250 are shown.
Line ID 169C03
Locus At1g11250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3768727
Primers used for PCR GJ01 - 8474, show primer details
   
Sequence Name 15-K026766-022-169-C03C-8409
GenBank Accession LN492814 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K026766-022-169-C03C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGGAAGTTTACTCTACC
ATTTTAAATTACCAATTGTCATTTTAGCCATTTTCATCATATATCATTTTTACTCTGTCT
TTATTTTCTTTCTCTGGCTTAATTATGTACAGAAGTTTTTTTTTTGGGGTTTTATGTGTT
TTTTTCTTTGATTTTGTTTTTGTTCAAAAGCAATTGATTATGATGATGAACATCACCATC
GTCGTCCTCTGCATGAGTAAGAGGAAGAAGATGCTAGGAAGTTTTTTTTTTGTTTTATTT
TGAGGAGGGAAAAAAAAAAACTTTTAGTTTTGGGGGAAGGTTTATTTTGTTTTGTTAGAA
ACGAGAGACACAAGCCACTTTTGGTAATTGTCCAAATTTAGTCAAGGGCATAACTTTTTC
TTAACCAAATTAGCTCCATTTAGTCAAAGACAATATTACCCTTATAAGGGGCCAAGCCTT
AAAGCCTAAAGCCCAATTAAAAGGCCATTTTTTCTGCTTCCGGCTT
   
Sequence Name 16-K026766-022-169-C03C-GJ01
GenBank Accession LN493282 [GenBank]
Sequencing primer gene specific primer, GJ01: 5'-TATGAAGAAGAAGAAGCAGGAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K026766-022-169-C03C-GJ01
AAAAAATGGCCTTTTAATTGGGCTTTAGGCTTTAAGGCTTGGCCCATTATAAGGGTAATA
TTGTCTTTGACTAAATGGAGCTAATTTGGTTAAGAAAAAGTTATGCCATTGACTAAATTT
GGACAATTACCAAAAGTGGCTTGTGTCTCTCGTTTCTAACAAAACAAAATAAACCTTCCC
ACAAAACTAAAAGTTTTTCTTTTTCACTCATCAAAATAAAACAAAAAAAAAACTTCCTAG
CATCTTCTTCCTCTTACTCATGCAAAGGACGACAATGGTGATGTTCATCATCATAATCAA
TTGCTTTTGAACAAAAACAAAATCAAAGAAAAAAACACATAAAACCCCAAAAAAAAAACT
TCTGTACATAATTAACCCAGAGAAAGAAAATAAAGACGGAGTAAAAATGATATATGATGA
AAATGGCTAAAATGACAATTGGTAATTTAAAATGGTAAAGTAAACTTCCAGGATATATTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCACCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAAATGTCC
GCAGCGTTTTTATAAAATGAAAGTACTTTTTGATAAAACGACAAA

Last Updated on 06.12.2018 16:11