Sequences from confirmation amplicon


Confirmation sequences for line 169D09 and hit At3g56740 are shown.
Line ID 169D09
Locus At3g56740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21016204
Primers used for PCR 03ht - 8474, show primer details
   
Sequence Name 38-K112689-0022-169-D09C-03ht
GenBank Accession LN503038 [GenBank]
Sequencing primer gene specific primer, 03ht: 5'-CCTGAGAACAAGATAAAAACCTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K112689-0022-169-D09C-03ht
TTTTGTAGTAAAGAAATAGTGAAATTTAAATATAAAAAACGAAAAAAAGAATCATCACCA
GTAAACCTTTCTAGAAACCAGTTTTCCAAGTGAACAAGAAAAACATAAAACACATGAGAA
TATATCTAAGAAGCAAAGAGAGTCGAGCTACAAGCATCAGTTACCGAGTACTTATTTGAG
CCAATCTGTCTCTCAAAAACTCGGAAGTAGTATAGCAAATACAATCCGAACATCAATTCT
GGTGTGGAGGAGAAGGCAAAAGTGGACATGATTAACTTCCATATCCGAAATTTCTCAAAA
ATGTCCTGCAGCGTGTTTGGAAGAAGATTAAATAGAGAAATAGTATTATGAGATATCAGA
TTTACATAACAAGTAGAAATAACTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 06-K112688-0022-169-D09C-8409
GenBank Accession LN488886 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K112688-0022-169-D09C-8409
TTGAAGTTATTTCTACTTGTTATGTAAATCTGATATCTCATAATACTATTTCTCTATTTA
ATCTTCTTCCAAACACGCTGCAGGACATTTTTGAGAAATTTCGGATATGGAAGTTAATCA
TGTCCACTTTTGCCTTCTCCTCCACACCAGAATTGATGTTCGGATTGTATTTGCTATACT
ACTTCCGAGTTTTTGAGAGACAGATTGGCTCAAATAAGTACTCGGTAACTGATGCTTGTA
GCTCGACTCTCTTTGCTTCTTAGATATATTCTCATGTGTTTTATGTTTTTCTTGTTCACT
TGGAAAACTGGTTTCTAGAAAGGTTTACTGGTGATGATTCTTTTTTTCGTTTTTTATATT
TAAATTTCACTATTTTCTTTTACTTACAAAAGTTAAATCAAAGGGTGTTGTTCCTGCTGC
AGGTTTTTATCTTGTTCTCAGGA

Last Updated on 06.12.2018 16:11