Sequences from confirmation amplicon


Confirmation sequences for line 169D12 and hit At2g30942 are shown.
Line ID 169D12
Locus At2g30942 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13171337
Primers used for PCR BL46 - 8474, show primer details
   
Sequence Name 26-K104985-0022-169-D12C-BL46
GenBank Accession LN497918 [GenBank]
Sequencing primer gene specific primer, BL46: 5'-GAAAGATGCCTGTGGAATAAGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K104985-0022-169-D12C-BL46
CACGCAAGAGGCTTATCAAGAAAGTGATTTCAAATGTTTCTTCTGAGCATACTTAACACA
CAACCTAAGTTCAAATCCCCAAAGAATCTGTTTGAAGTCGCTCTGCTCTGTGTTAAGAGA
ATTGAATGCATAAGATAGAGCAAACTCTAGCAACCCTCTAAATCCGTTATAAGCACAGAG
ATCAAAGGAACATAACATTCAATGTAGTTCATTAACAATCGGATGATATAGAATATCAAA
TGTGATGTAAAATCCACAAACCCTCTTGATATCTTTAATCAATAGAACATAGATACATAT
ACACACACTAACTAGAGATAGAAACTAGTAAAACATATAAGCAAAACAAGGATTTGAGAT
GGTGACAAAACCCAAACCTAAATCAAACTAAAGCTATACATTAAGACCATTAAAGTTACC
TCTTGCAAAGCTCGGAAAAGTAAATGAAAAAAACAGAAATGAAAGAAAAAGAAAAACAGA
AAGCAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGA
   
Sequence Name 10-K104986-0022-169-D12C-8409
GenBank Accession LN490645 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K104986-0022-169-D12C-8409
TTGAATAATTGCTTTCTGTTTTTCTTTTTCTTTCATTTCTGTTTTTTTCATTTACTTTTC
CGAGCTTTGCAAGAGGTAACTTTAATGGTCTTAATGTATAGCTTTAGTTTGATTTAGGTT
TGGGTTTTGTCACCATCTCAAATCCTTGTTTTGCTTATATGTTTTACTAGTTTCTATCTC
TAGTTAGTGTGTGTATATGTATCTATGTTCTATTGATTAAAGATATCAAGAGGGTTTGTG
GATTTTACATCACATTTGATATTCTATATCATCCGATTGTTAATGAACTACATTGAATGT
TATGTTCCTTTGATCTCTGTGCTTATAACGGATTTAGAGGGTTGCTAGAGTTTGCTCTAT
CTTATGCATTCAATTCTCTTAACACAGAGCAGAGCGACTTCAAACAGATTCTTTGGGGAT
TTGAACTTAGGTTGTGTGTTAAGTATGCTCAGAAGAAACATTTGAAATCACTTTCTTGAT
AAGCCTCTTGCGTTGTTATGCACTGCTTAATTATGTTAAATCCTTCTTATTCCACA

Last Updated on 06.12.2018 16:11