Sequences from confirmation amplicon


Confirmation sequences for line 169E08 and hit At1g64900 are shown.
Line ID 169E08
Locus At1g64900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24113482
Primers used for PCR ML49 - 8474, show primer details
   
Sequence Name 07-K100810-0022-169-E08C-8409
GenBank Accession LN489187 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K100810-0022-169-E08C-8409
CATTTACAATTGCTGATCCTATCTCCGCTCTGATCCATCGTCACCCTCCGCATCACTTCC
CGCCCCGCCATATTCGTTGCCGATCGTTCCCTAACTCACGAGGCTCTCGTCCTGAACGGC
GCCGTCTATGCGGACCGTCCACCACCTGCTGTAATTAGCAAGATAGTTGACGAACACAAC
ATCAGCTCCGGGTCATACGGAGCCACGTGGCGGCTTCTCCGTCGAAACATCACCTCTGAG
ATTCTTCACCCCTCGCGCGTGAGATCTTACTCCCACGCTCGTCACTGGGTTCTTGAGATC
CTCTTTGAACGGTTCCGTAACCATGGAGGCGAAGAACCGATCGTCCTCATCCACCATCTC
CACTATGCAATGTTTGCGCTTCTTGTCCTTATGTGTTTTGGAGATAAGCTTGATGAGAAG
CAAATCAAAGAAGTGGAGTTCATTCAAAGACTACAGCTCCTTAGCTTGACCAAATTTAAC
ATCTTCAACATCTGGCCTAAGTTCACCAAACTGATTCTGCGTAAGAGATGGCAAGAATTT
CTCCAGATACGGAGACAGCAGCGGGATGTTTTGCTTCCCCTGATTCGTGCCCGGAGGAAG
ATCGTTGAAGAGAGAAAGAGATCGGAGCAAGAAGACNAGAAAGACTATGTGCAATCATAT
GTTGATACTCTACTCGATCTAGAGCTTCCAGA
   
Sequence Name 07-K100824-0022-169-E08C-ML49
GenBank Accession LN489189 [GenBank]
Sequencing primer gene specific primer, ML49: 5'-CGTTCAACTTCCTATTTTCCTCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K100824-0022-169-E08C-ML49
ACATATGATTGCACATAGTCTTTCTTGTCTTCTTGCTCCGATCTCTTTCTCTCTTCAACG
ATCTTCCTCCGGGCACGAATCAGGGGAAGCAAAACATCCCGCTGCTGTCTCCGTATCTGG
AGAAATTCTTGCCATCTCTTACGCAGAATCAGTTTGGTGAACTTAGGCCAGATGTTGAAG
ATGTTAAATTTGGTCAAGCTAAGGAGCTGTAGTCTTTGAATGAACTCCACTTCTTTGATT
TGCTTCTCATCAAGCTTATCTCCAAAACACATAAGGACAAGAAGCGCANACATTGCATAG
TGGAGATGGTGGATGAGGACGATCGGTTCTTCGCCTCCNTGGTTACGGAACCGTTCAAAG
AGGATCTCAAGAACCCAGTGACGAGCGTGGGAGTAAGATCTCACGCGCGAGGGGTGAAGA
ATCTCAGAGGTGATGTTTCGACGGAGAAGCCGCCACGTGGCTCCCTATGACCCGGAGCTG
ATGTTGTGTTCGTCAACTATCTGGCTNATTACAGCAGGTGGTGGACGGTCCGCATAGA

Last Updated on 06.12.2018 16:11