Sequences from confirmation amplicon


Confirmation sequences for line 169H08 and hit At3g27580 are shown.
Line ID 169H08
Locus At3g27580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:10218567
Primers used for PCR DE13 - 8474, show primer details
   
Sequence Name 43-K019240-022-169-H08C-8409
GenBank Accession LN504682 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K019240-022-169-H08C-8409
TAGATTTCCCGGACATGAAGCCATTTACATGTAGATTTCTTTTTCTTTGTCTTGTTTGTA
GATTTTATGTTGCTGAAGTTCTTCTTGCTATGGAGTATCTACATATGCTTGGGATCATAT
ACAGAGACCTTAAGCCTGAGAATGTTTTAGTTCGTGAAGATGGACATATAATGCTATCTG
ATTTCGACCTCTCTTTGAGATGTGCAGTCTCTCCAACGTTGGTTCGGTTCGCAGCAATCA
CGCTTGAGTCCAAAAGCTCAAGCTACTGTATCCAACCAACATGTGTAGACCAATCGAGCT
GCATTGTTCAGCCTGACTGCATCCAGCCAGTGTGTTTCACTCCTCGGTTCTTGTCTAAAG
GAAAGCACAGGAAGAAGTCGAACGATATGAGCCGCCAAATCAGACCGCTTCCTGAGCTGA
TAGCTGAACCGACTAGCGCAAGGTCAATGTCGTTTGTTGGTACACACGAGTACCTAGCAC
CAGAGATTATCAAAGGAGAAGGTCATGGAAGCGCGGTTGACTGGTGGACATTCGGGATAT
TCCTCTATGAG
   
Sequence Name 44-K019240-022-169-H08C-DE13
GenBank Accession LN505034 [GenBank]
Sequencing primer gene specific primer, DE13: 5'-CCCAAATAGAAGCTCATAGAGGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K019240-022-169-H08C-DE13
TATCCCAGAATGTCCACCAGTCAACCGCGCTTCCATGACCTTCTCCTTTGATAATCTCTG
GTGCTAGGTACTCGTGTGTACCAACAAACGACATTGACCTTGCGCTAGTCGGTTCAGCTA
TCAGCTCAGGAAGCGGTCTGATTTGGCGGCTCATATCGTTCGACTTCTTCCTGTGCTTTC
CTTTAGACAAGAACCGAGGAGTGAAACACACTGGCTGGATGCAGTCAGGCTGAACAATGC
AGCTCGATTGGTCTACACATGTTGGTTGGATACAGTAGCTTGAGCTTTTGGACTCAAGCG
TGATTGCTGCGAACCGAACCAACGTTGGAGAGACTGCACATCTCAAAGAGAGGTCGAAAT
CAGATAGCATTATATGTCCATCTTCACGAACTAAAACATTCTCAGGCTTAAGGTCTCTGT
ATATGATCCCAAGCATATGTAGATACTCCATAGCAAGAAGAACTTCAGCAACATAAAATC
TACAAACAAGACAAAGAA

Last Updated on 06.12.2018 16:11