Sequences from confirmation amplicon


Confirmation sequences for line 170A07 and hit At1g17260 are shown.
Line ID 170A07
Locus At1g17260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5904218
Primers used for PCR 02pd - 8474, show primer details
   
Sequence Name 46-K115005-0022-170-A07C-02pd
GenBank Accession LN505951 [GenBank]
Sequencing primer gene specific primer, 02pd: 5'-TACTTGACTATTAGCGAGGGCAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K115005-0022-170-A07C-02pd
CAGGGATTCCACATAAAACCTAAGAATTTCACAAATCTGTTCTCCTGTATCATTGTTGTT
TTAAATGGCGGTTAGCACAAGATAATAGAGACAATTGGCAGGAACACTAAGAAGAATGTC
ATGTTTATTACCTGTTTCTCTTCAAGTCTGTTAGGACCAAATATCTTCAATCTCTCTTCA
GCATCTCCAGATAAAAGCCCTTGAGGCGATGTTCTTAGGTATTCAAAAACCTCCTCCAAC
GGCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCA
   
Sequence Name 14-K115004-0022-170-A07C-8409
GenBank Accession LN492630 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K115004-0022-170-A07C-8409
TCATTGCCGTTGGAGGAGGTTTTTGAATACCTAAGAACATCGCCTCAAGGGCTTTTATCT
GGAGATGCTGAAGAGAGATTGAAGATATTTGGTCCTAACAGACTTGAAGAGAAACAGGTA
ATAAACATGACATTCTTCTTAGTGTTCCTGCCAATTGTCTCTATTATCTTGTGCTAACCG
CCATTTAAAACAACAATGATACAGGAGAACAGATTTGTGAAATTCTTAGGTTTTATGTGG
AATCCCTTGTCATGGGTTATGGAAGCTGCTGCATTGATGGCCATTGCCCTCGCTAATAGT
CAAGT
Primers used for PCR 11vg - 8474, show primer details
   
Sequence Name 47-K117737-0022-170-A07C-11vg
GenBank Accession LN506321 [GenBank]
Sequencing primer gene specific primer, 11vg: 5'-TACTGTTCGCAAACTCTCTTATGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K117737-0022-170-A07C-11vg
ATCTCCTCTTCTCCACACTCTATCAACTGGTCAGCCATGGAATGGTCGTTTCAGTTTCAA
TATTCCTGGATTCTTTTTAAGGATTCCTGTTTCTCTTCTGTTCCTGGTATATTCTTAACG
ACGAAATTAGTATCGGATCCTGGTAATACATTTTGAAGCTTTTAAGTATGGCCGAGGATT
TGGACAAGCCATTGCTGGATCCTGATACTTTCAACAGAAAAGGAATTGATTTGGTAAATG
TTCCTTTTAACTGTATACTCTTATTAGTACTTGAATTCTTAGTGGCAAAAAAGGGTTTTG
ACCCTTCTTGAGCTTGACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATTATAAAA
   
Sequence Name 15-K117736-0022-170-A07C-8409
GenBank Accession LN493158 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K117736-0022-170-A07C-8409
GCCATTTACAATTGAATATATCCTGTCAAGCTCAAGAAGGGTCAAAACCNCTTTTTTGCC
ACTAAGAATTCAAGTACTAATAAGAGTATACAGTTAAAAGGAACATTTACCAAATCAATT
CCTTTTCTGTTGAAAGTATCAGGATCCAGCAATGGCTTGTCCAAATCCTCGGCCATACTT
AAAAGCTTCAAAATGTATTACCAGGATCCGATACTAATTTCGTCGTTAAGAATATACCAG
GAACAGAAGAGAAACAGGAATCCTTAAAAAGAATCCAGGAATATTGAAACTGAAACGACC
ATTCCATGGCTGACCAGTTGATAGAGTGTGGAGAAGAGGAGATTCTCCTGCAAGTCTCAA
TTGGTATCACCATTATTGGAAGCATAAGAGAGTTTGCGAACAGTAATGNNNNNNNCTCCG
GGACTTCTCGGTGGTTTTCCCCTTTTGGTGCGCCTTGAAAA

Last Updated on 06.12.2018 16:11