Sequences from confirmation amplicon


Confirmation sequences for line 170C06 and hit At1g47760 are shown.
Line ID 170C06
Locus At1g47760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:17572511
Primers used for PCR ML48 - 8474, show primer details
   
Sequence Name 06-K100810-0022-170-C06C-8409
GenBank Accession LN488723 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K100810-0022-170-C06C-8409
TTTTCAGACGCCGAATGGCATTTTTAGAAGGCTGATGAACTCGCGAAGTTGTGTAATGTA
GAGATTGCTGTCTTGGTCATCTCCCCCACTAATATACCATACACATACGGTTATCCATGC
TTCAACGATGTGGTTGAGCGTATTCAAAATCCTAGTGCTTCGTCCAAACTCAGGAGTCTC
ATGAAAGAATTGGAACAAATCAAAGAGTTTCAGGAGGTATGTTTCCCATCATTAATATAA
TCTCATCCGTTAGTTTTCCTTCTTCTTCGTTCTCAACATATAGATTACAAAGATGGGTTT
GCTATTATACACAGAATTAAATAATCATTATTTATTTTATATTATATAATATTTTTTTTG
TCAATTTTAGGATTTGAGGAAGAAACAGCAAAGAAACCTTGAAAAGTCCAATATGAAAGA
GAATGTAGATCTGAAATTGGAGGATTAAGTTGC
   
Sequence Name 06-K100824-0022-170-C06C-ML48
GenBank Accession LN488725 [GenBank]
Sequencing primer gene specific primer, ML48: 5'-AAGCAACTAAGTCCTCCAATTTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K100824-0022-170-C06C-ML48
TTTCAGGTTTCTTTGCTGTTTCTTCCTCAATCCTAAAATTGACAAAAAAAATATTATATA
ATATAAAATAAATAATGATTATTTAATTCTGTGTATAATAGCAAACCCATCTTTGTAATC
TATATGTTGAGAACGAAGAAGAAGGAAAACTAACGGATGAGATTATATTAATGATGGGAA
ACATACCTCCTGAAACTCTTTGATTTGTTCCAATTCTTTCATGAGACTCCTGAGTTTGGA
CGAAGCACTAGGATTTTGAATACGCTCAACCACATCGTTGAAGCATGGATAACCGTATGT
GTATGGTATATTAGTGGGGGAGATGACCAAGACAGCAATCTCTACATTACACAACTTCGC
GAGTTCATCAGCCTTCTTAAAAATGCCATTTCGGCGTCTTGAAAACGTCGCAAGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGA

Last Updated on 06.12.2018 16:11