Sequences from confirmation amplicon


Confirmation sequences for line 170D03 and hit At1g34210 are shown.
Line ID 170D03
Locus At1g34210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:12460023
Primers used for PCR NH61 - 8474, show primer details
   
Sequence Name 16-K102660-0022-170-D03C-8409
GenBank Accession LN493386 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K102660-0022-170-D03C-8409
AGTCTCTAGAAATAAGTAGTTATGCTACACATAGTCAATGCCATCAACTTTTCGTTTATC
TACAGAGTAACGATTCATCTTCCATTTAGATATCAAAGCAAAAACTTACAAGTACTGCAA
GTTCTTGAGCTGACCTAGCTGAGGAACCAACTGACCAGACAAGTCTGCATTCCCAAGATC
GCTGAAGAAGCAACAACAAACAGAGATTAAATAAGCTAAACAAAGAACATTGGTCAGGTC
AGGTATAAGCAGAGAGACACTCACACTCTTATAACACTGTTCTCGTTGTTACACGTTACG
TGAAACCAAGTACACGGATTAACAAGCGTAGGATCCCAGCTTTGCAAGACATTATTTGGA
TCAACTAGATNAGCTCTCAAACGNTGCAGTG
   
Sequence Name 35-K102661-0022-170-D03C-NH61
GenBank Accession LN501787 [GenBank]
Sequencing primer gene specific primer, NH61: 5'-CACTGCACAGTTTGAGAGCTAATC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K102661-0022-170-D03C-NH61
AGCTGGATCCTACGCTTGTTAATCCGTGTACTTGGTTTCACGTAACGTGTAACAACGAGA
ACAGTGTTATAAGAGTGTGAGTGTCTCTCTGCTTATACCTGACCTGACCAATGTTCTTTG
TTTAGCTTATTTAATCTCTGTTTGTTGTTGCTTCTTCAGCGATCTTGGGAATGCAGACTT
GTCTGGTCAGTTGGTTCCTCAGCTAGGTCAGCTCAAGAACTTGCAGTACTTGTAAGTTTT
TGCTTTGATATCTAAATGGAAGATGAATCGTTACTCTGTAGATAAACGAAAAGTTGATGG
CATTGACTATGTGTAGCATAACTACTTTATTTCTAGAGACTTGTAGTTTCTCATACATGA
TATAGGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAA
AACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATT
CGGAAATCTTTATTTCGACGTGTCTACATTCA
Primers used for PCR MN61 - 8474, show primer details
   
Sequence Name 38-K118534-0022-170-D03C-MN61
GenBank Accession LN503156 [GenBank]
Sequencing primer gene specific primer, MN61: 5'-CTAGAGAATCTGGAATTGGACCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K118534-0022-170-D03C-MN61
TTTGTCGATTCCCAAGATCGCTTGGAACCGGCCCGGTTATGTTATTACTATAAAGCTCCC
TGCCATTAAGAGTATCACACCCTCAGAAGAAAATTAACAATGTACAATGACAAATGGGTA
TTGAATAAGTCCTAATAAAATGAAAAAACAAGATTCAGGATATATTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTA
   
Sequence Name 17-K118533-0022-170-D03C-8409
GenBank Accession LN494068 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K118533-0022-170-D03C-8409
TACATTGAATATATCCTGAATCTTGTTTTTTCATTTTATTAGGACTTATTCAATACCCAT
TTGTCATTGTACATTGTTAATTTTCTTCTGAGGGTGTGATACTCTTAATGGCAGGGAGCT
TTATAGTAATAACATAACCGGGCCGGTTCCAAGCGATCTTGGGAATCTGACAAACTTAGT
GAGCTTGGATCTTTACTTGAACAGCTTCACTGGTCCAATTCCGAATTCTCTAGAANNNNG
NNNGAGTATTCCCCTTTTTTTTTTCATTCAAAAATGTAGATCCCCGCAATAATAAAAA
Primers used for PCR 31wp - 8474, show primer details
   
Sequence Name 39-K118534-0022-170-D03C-31wp
GenBank Accession LN503521 [GenBank]
Sequencing primer gene specific primer, 31wp: 5'-AATGGTCAACTGCTGATAATTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K118534-0022-170-D03C-31wp
GCAGCGTTATATCAATTGAGTAAATTTACTTAAGAGTTAAGATGATCAAAAGAAACTTAG
GTTCAGACTTTAAATTTTATTGCCACTTGTGCTTAATCTTAACTTATAGCCTCAGTTCCC
ATAATGCGAGTAACTAATGTTTGCATAGTATGGATTCAATCAAACCTGATGGGAGTGAAG
AGCGAGAAGGAACCATTATCAGGAACAGATCCGGATAATCGGTTGTTCGACAGATCCCTG
CAGTTTGCAAGATAATAAGGAAAACTCAGGTGATCCATTAAGAGTGAGAAGTTCAGAAGA
GCAGAAAGTAAAATTGAAAAGTAATACTCACAAAACTTGAAGGGTCATGATATTAGTCAA
TGACATGGGAATTGGTCCGGTGAGACTGTTATTGTTGAGCCGACTATCCTTGCAACAAAA
ACTCAGACCACATTACCATCTTCCCATACCAGAGTTCAATCAAACAAGTTTATTCAGTGT
TTTGAAAGACAAAGCCAATTTCTTTTACTTCTATTCAAATGTCCAACAAGTAATAACATT
TAGCCAAAAGAGAATTTAATCCTCAGTAAATTTCTAATATAGCAAAACATTTAGCCAGTG
CATGATGAGAAGAGCAGACTAAGTGTTCTTACAGAAAGCGAAGCTTGAATAGCTTTCCTA
GAGAATCTGGAATTGGACCAGTGAAGCTGTTCAAGTAAAGATCCAAGCTCACTAAGTTTG
TCAGATTCCCAAGATCGCTTGGAACCGGCCCGGTTATGTTATTACTATAAAGCTCCCTGC
CATTAAGAGTATCACACCCTCAGAAGAAAATTAACAATGTACAATGACAAATGGGTATTG
AATAAGTCCTAATAAAATGAAAAAACAAGATTCAGGATATATTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGAATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTTATTTACCAATTTTTTTTCATTTCAAAATGT
   
Sequence Name 18-K118533-0022-170-D03C-oriL
GenBank Accession LN494518 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K118533-0022-170-D03C-oriL
CATTTACATTGAATATATCCTGAATCTTGTTTTTTCATTTTATTAGGACTTATTCAATAC
CCATTTGTCATTGTACATTGTTAATTTTCTTCTGAGGGTGTGATACTCTTAATGGCAGGG
AGCTTTATAGTAATAACATAACCGGGCCGGTTCCAAGCGATCTTGGGAATCTGACAAACT
TAGTGAGCTTGGATCTTTACTTGAACAGCTTCACTGGTCCAATTCCAGATTCTCTAGGAA
AGCTATTCAAGCTTCGCTTTCTGTAAGAACACTTAGTCTGCTCTTCTCATCATGCACTGG
CTAAATGTTTTGCTATATTAGAAATTTACTGAGGATTAAATTCTCTTTTGGCTAAATGTT
ATTACTTGTTGGACATTTGAATAGAAGTAAAAGAAATTGGCTTTGTCTTTCAAAACACTG
AATAAACTTGTTTGATTGAACTCTGGTATGGGAAGATGGTAATGTGGTCTGAGTTTTTGT
TGCAAGGATAGTCGGCTCAACAATAACAGTCTCACCGGACCAATTCCCATGTCATTGACT
AATATCATGACCCTTCAAGTTTTGTGAGTATTACTTTTCAATTTTACTTTCTGCTCTTCT
GAACTTCTCACTCTTAATGGATCACCTGAGTTTTCCTTATTATCTTGCAAACTGCAGGGA
TCTGTCGAACAACCGATTATCCGGATCTGTTCCTGATAATGGTTCCTTCTCGCTCTTCAC
TCCCATCAGGTTTGATTGAATCCATACTATGCAAACATTAGTTACTCGCATTATGGGAAC
TGAGGCTATAAGTTAAGATTAAGCACAAGTGGCAATAAAATTTAAAGTCTGAACCTAAGT
TTCTTTTGATCATCTTAACTCTTAAGTAAATTTACTCAATAGACATACTACATTTTCCAA
ATCTATTACTTTAATGGTGGATATGGAAATATC

Last Updated on 06.12.2018 16:11