Sequences from confirmation amplicon


Confirmation sequences for line 170G02 and hit At3g12280 are shown.
Line ID 170G02
Locus At3g12280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3916082
Primers used for PCR WG02 - 8474, show primer details
   
Sequence Name 02-K020358-022-170-G02C-oriL
GenBank Accession LN486845 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K020358-022-170-G02C-oriL
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATACATATGGGCAGAGTATG
TGTGCTCTTCGTGTGACGTCATTTGTTATATCCCTATCACATGATTTAAGGAAATGTTCC
AACCCAGGAGAAGGTTTTGGCAGAAGCGGGGATATGACAGTCCTGAGCCACTTGGCAGTT
GTCATTGCTGTGCTCACTGGTGTTGCTGCCAACTTGGTAGCACCGCTAATACCATTTGTC
TTAGCAGCAGGCGACTTATGAGGAGAAAGTGGGCTTATAAATGTCCTTGCAGGTGAGCTC
AAAGCATCAATTTTCCTCTTAACACCAGTAATATTAACAGCTCCTGCAGATAAGCTTCCA
GATCCAAGTAAGCTATCCTCTTCATTGATGAATACCCTCTCATCAAGTTCGCCTTTACCA
TCATAGTAATCCTTTTCAAGTGTAATTAAGCTAGTTGAGATGGACGTCTCTTCCAGTAAA
TCCTCAAAGTAGGTCAAGCCATCTGCAGTAAAACACCATATAGATTCGAAGAACAATCAG
GCAACTGTTCAATGAACTCACATGATTCTTTAATTGAAGAAGGAAAATGATTTTAGACCA
ACCTGGGTCAATATTATCTAGCTTGTCAGTTTGGCACTCAGATGCTGGAGA
   
Sequence Name 06-K020358-022-170-G02C-WG02
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, WG02: 5'-CCATACTGAAGAAAAAGCCATCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K020358-022-170-G02C-WG02
TGGTCTTCCAAACCATATGGGGGGTTTCTGCCGAAGGGGTGGCCCCCCTTTTGGGGTTTC
TGGGAGAAAAAACCCTCTCCACCAACTTTATTTCCCCTTGAAAAGGGTTCTTTTATTGGG
AAAGGGGACTTTTTTAGAGGGGGTTTCTCCCTGAAAAAGAAAACTTTCTTTGGTCTGGGA
AATTTTTTTCCCGAGGGGGTTATATTACTGGGGGGTAAAAGAAAAATTATGGTTTTTGCT
CCCCCCCCAGGGGATTTTTTAACCCCCCTTTTCCCCCTAAAAAGCCCGCTGGTTAAAAAA
AAGGGGTTTTCGGGGGTTCCCAAATGGGGGCCACCCCCCCGGGGGCCCCCATGACCACCT
CCCAAAGGG
Primers used for PCR WG01 - 8474, show primer details
   
Sequence Name 59-K020358-022-170-G02C-8409
GenBank Accession LN509864 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K020358-022-170-G02C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTACATGTCCAATAT
ATGGCTTCATGAAGCCATATACTCATTGCTGATCAATGTGGAGGTGGAAGTTTGCAAGCT
GTTGACCTGATGGATGACATATGGGCAGAGCAGCGCAGATTAGAAGCTTGTAAGTTATAC
TACAGAGTTCTTGAGGCAATGTGTAAAGCAGAAGCTCAGATTTTGCATGCAAATAATCTG
AACTCTTTATTGACAAATGAGAGGTTCCATAGATGCATGCTTGCTTGCTCAGCTGAATTG
GTACTGGCTACCCACAAAACAATTACAATGTTGTTCCCAGCTGTTCTGGAGAGGACTGGG
ATCACAGCCTTTGATCTCAGCAAGGTAATTGAGAGTTTCATACGACATGAAGATTCTCTG
CCTAGAGAGTTGAGACGACATCTGAATTCACTGGAGGAACGGCTTCTAGAGAGTATGGTA
TGGGAGAAAGGCTCTTCAATGTACAATTCTCTGATTGTTGCCAGGCCATCGCTTGCATTG
GGGATAAATCAGCTCGGTTTACTAGCTGAACCAATGCCATCTCTGGATGCAATCGCAGCA
CTTATTAATTTCTCTGACGGAGCAAATCATGCATCATCTGTACAAAAGCATGAAACTTGT
CCAGGTAGTTTTATTTGTTTCTGAATTAAAGCAGTT
   
Sequence Name 63-K020358-022-170-G02C-WG01
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, WG01: 5'-TCATACCATTAACAACAGGTTGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K020358-022-170-G02C-WG01
ACCTTTTTCCCTAACAAAATCTCTTAAAAACGGTCCCCCCCCGGAATTAAAAGGCGGCTC
CACCCTCTTTGGGGAAAAAATTCCTGGCGGATGAAAAACCCCAATACCCTGCTGGGAAAA
AAAGGGGGGGTTCCCCGCCTCTCCCCAAAAAATGGGGAAAACAATGGAAATGGTTTGTGG
GGGGGCCCGACCCATTTTTCTGAGCAAACAAACATGGCTCTTTTGGAACCCCCCTTTGTC
CATAAAAAAGTCCGAATATTTTGCTGGGAAAAAT

Last Updated on 06.12.2018 16:11