Sequences from confirmation amplicon


Confirmation sequences for line 170G10 and hit At1g62570 are shown.
Line ID 170G10
Locus At1g62570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23170958
Primers used for PCR KU73 - 8474, show primer details
   
Sequence Name 13-K103353-0022-170-G10C-KU73
GenBank Accession LN492001 [GenBank]
Sequencing primer gene specific primer, KU73: 5'-TACATTAATTTGCAGGTGGTGGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K103353-0022-170-G10C-KU73
GTAGAGACATATCTAAGTCGCAAAAGAAGTTCATATCGCATCTAGAGCAAGTAAATCCAA
CACTTTCGAAAAACGTCCTGTACCTAATAACAATCTCTGGATGCACTCTGAGGTAAAAAC
TATTTTCTTATAATGGTTAATCTTGATAAGAAAAAATTCGAAACAAATTATAAAATTCTT
GCTTCTATTAATTATTTTATTTTTTTGATATCTTGCAGATAGACACCGCCCACGAGGATG
GTACCATTGTTTTTAAAAATGGGAAGGTGGTACATGCTGATACCATTGTCCATTGTACCG
GGTAAACCTAAGAGAAAAGAGCAAAAGAAAGAGTAATTACCAATTTTTTCAATTGATCCC
CGCCAAATGAAAATCTACATGTCCGACTAAAGGAGGCGGCATGGAAAATCACGCGAGTAT
CATGGAAAATCTCCATGTAGATTTCTCCAGGATATATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 29-K103354-0022-170-G10C-8409
GenBank Accession LN499246 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K103354-0022-170-G10C-8409
GCATTTACAATTGAATATATCCTGGAGAAATCTACATGGAGATTTTCCATGATACTCGCG
TGATTTTCCATGCCGCCTCCTTTAGTCGGACATGTAGATTTTCATTTGGCGGGGATCAAT
TGAAAAAATTGGTAATTACTCTTTCTTTTGCTCTTTTCTCTTAGGTTTACCCGGTACAAT
GGACAATGGTATCAGCATGTACCACCTTCCCATTTTTAAAAACAATGGTACCATCCTCGT
GGGCGGTGTCTATCTGCAAGATATCAAAAAAATAAAATAATTAATAGAAGCAAGAATTTT
ATAATTTGTTTCGAATTTTTTCTTATCAAGATTAACCATTATAAGAAAATAGTTTTTACC
TCAGAGTGCATCCAGAGATTGTTATTAGGTACAGGACGTTTTTCGAAAGTGTTGGATTTA
CTTGCTCTAGATGCGATATGAACTTCTTTTGCGACTTTAGATATGTCTCTACTGATATCG
GCTCCACTCGCAAAATTCCCGATTACTACCACCACCTGCAATTTAATGT

Last Updated on 06.12.2018 16:11