Sequences from confirmation amplicon


Confirmation sequences for line 171F04 and hit At5g24240 are shown.
Line ID 171F04
Locus At5g24240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8231462
Primers used for PCR 04bq - 8474, show primer details
   
Sequence Name 24-K107934-0022-171-F04C-04bq
GenBank Accession LN497021 [GenBank]
Sequencing primer gene specific primer, 04bq: 5'-AAACTCATCTTCGGTTGTCTCTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K107934-0022-171-F04C-04bq
ATTGCTCGATCCAGATTCTTCCTTGAGTGTTTCTCTGCACATTATGCTTCCGATGGTAAA
AGGTGTGAGACCCTTTGCAGAACCTTTCTTTAGAAGCATGGTTGAAATACGGAGGACACG
GGTGCATGAAGGTGGAATCTCCCAACCATGGAATCTCAGAAGTTCGATGTCTTTCTCGGG
GTCCAAGGATTTGATGTATTCCAATGTCTCTGAAGAGTATGGCTCTTTTGCTTGAGGCCA
GTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCAGCGTTATTA
   
Sequence Name 08-K107933-0022-171-F04C-8409
GenBank Accession LN489762 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K107933-0022-171-F04C-8409
ATTTACATTGAACTGGCCTCAGCAAAAGAGCCATACTCTTCAGAGACATTGGAATACATC
AAATCCTTGGACCCCGAGAAAGACATCGAACTTCTGAGATTCCATGGTTGGGAGATTCCA
CCTTCATGCACCCGTGTCCTCCGTATTTCAACCATGCTTCTAAAGAAAGGTTCTGCAAAG
GGTCTCACACCTTTTACCATCGGAAGCATAATGTGCAGAGAAACACTCAAGGAAGAATCT
GTGATCGAGCAAATCATACATGATGCAGAAGCCATAGTGCCCACAGAGACAACCGAAGAT
GAGTT

Last Updated on 06.12.2018 16:11