Sequences from confirmation amplicon


Confirmation sequences for line 173A03 and hit At3g09400 are shown.
Line ID 173A03
Locus At3g09400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2892157
Primers used for PCR BL54 - 8474, show primer details
   
Sequence Name 50-K104987-0022-173-A03C-BL54
GenBank Accession LN507161 [GenBank]
Sequencing primer gene specific primer, BL54: 5'-ATCGGATGAGAGGATTAGGAATTT-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K104987-0022-173-A03C-BL54
GATGTACGGCGATGTTCCGACGTAGTCTATTCTGAACATCTCTAGAAGCGCCTCGTTCCA
TTTTGGCTGAGGAAAGACATTGAACAAAACTATTATCAAATCGAATAAAAACAAACCAGG
TTTTGTAATAGTATTTTTCTGCATTTTGATATTTCTACAAACCTTGTAGACCTAATAATC
CAACCCTAAAAACTATAAAACATGCAAAATTTTGAAACTATCTGATTAAAATCATGAGTA
TATATATACCTGTTTAAGAAATCCAGCGCCAAATGCACGAGTGACTTTGAGATAACCTTT
CACTCTATTATTCTCTATCGCTAATATATCATCAGGATGTTCCTTCTTGATTCTTCTCAC
TTCCTGTCACATCAAAAGATCGTTAGTGTCTAATGTTCGCAAAACAAAACACTGAATCAG
GACTCGCAAAAAGATTCACCTCTTCAACACTTGTGCTATGTTCCTTATTGAGCTGAACAG
GGACCAGAAGACTCAGTCCTCTTTCGGTGATGAACAAGGTCTCTAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 34-K104988-0022-173-A03C-8409
GenBank Accession LN501408 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K104988-0022-173-A03C-8409
AGACCTTGTTCTCACCGAAAGAGGACTGAGTCTTCTGGTCCCTGTTCAGCTCAATAAGGA
ACATAGCACAAGTGTTGAAGAGGTGAATCTTTTTGCGAGTCCTGATTCAGTGTTTTGTTT
TGCGAACATTAGACACTAACGATCTTTTGATGTGACAGGAAGTGAGAAGAATCAAGAAGG
AACATCCTGATGATATATTAGCGATAGAGAATAATAGAGTGAAAGGTTATCTCAAAGTCA
CTCGTGCATTTGGCGCTGGATTTCTTAAACAGGTATATATATACTCATGATTTTAATCAG
ATAGTTTCAAAATTTTGCATGTTTTATAGTTTTTAGGGTTGGATTATTAGGTCTACAAGG
TTTGTAGAAATATCAAAATGCAGAAAAATACTATTACAAAACCTGGTTTGTTTTTATTCG
ATTTGATAATAGTTTTGTTCAATGTCTTTCCTCAGCCAAAATGGAACGAGGCGCTTCTAG
AGATGTTCAGAATAGACTACGTCGGAACATCGCCGTACATCACGTGCTCTCCGTCGCTAC
ACCACCATAGACTCTCATCACGTGACAAATTCCT

Last Updated on 06.12.2018 16:11