Sequences from confirmation amplicon


Confirmation sequences for line 173D05 and hit At5g02960 are shown.
Line ID 173D05
Locus At5g02960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:693220
Primers used for PCR 72lx - 8474, show primer details
   
Sequence Name 41-K117387-0022-173-D05C-72lx
GenBank Accession LN504234 [GenBank]
Sequencing primer gene specific primer, 72lx: 5'-TCTCTTGGGTTTTGAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K117387-0022-173-D05C-72lx
TATTTAGATGAATTTCTTATTGATTTTCTGAGTTTTACTATGGTTTATCGAGTGAAGTTG
TTCTCAAATGTTATTTTGTGATGATTTGTTAACAGTAAGACACGTGGTATGGGAGCTGGG
CGTAAGCTGAAGAGACTCCGAATTAACCAGAGGTGGGCTGACAAGCAGTACAAGAAGTCT
CACTTGGGAAATGAGTGGAAGAAGCCTTTTGCTGGTTCTTCTCACGCTAAAGGAATTGTT
CTTGAGAAAATGTAAGTCTGATATAACATTCAAGTTTACTTTTTCTTATAATTGATTTGT
TGAGTAGAGACATTGGTCGTTGATGAGACTCTTGTGTTTGATGATGCAGTGGTATTGAGG
CTAAGCAGCCTAACTCTGCTATCCGTAAGTGTGCTAGAGTTCAGCTTATCAAGAATGGAA
AGAAGATTGCTGCCTTTGTCCCCAATGATGGTTGTTTGAACTACATCGAGGAAAACGTAA
GTCACATGAAATGACTATTCAATCATCACACTATGGATTAGTTTCATTCATATGCTGATA
CATTTCTTGTTTATGGTGTTGCAGGATGAGGTCTTGATTGCTGGATTTGGTCGTAAGGGT
CACGCTGTGGGAGATATTCCCGGAGTTAGGTTCAAGGTCGTGAAGGTTTCTGGTGTTTCA
CTCTTGGCCCTCTTCAAGGAAAAGAAGGAGAAGCCAAGATCTTAAGCAACCCGTACAAAA
AGCTTCAAGCTTTTGATATGGTTTTTGATAGTTCAGTTTAAGTCTACATGGATATATTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCATTCAAA
   
Sequence Name 14-K117386-0022-173-D05C-8409
GenBank Accession LN492689 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K117386-0022-173-D05C-8409
ATTTACATTGAATATTCCATGTAGACTTAAACTGAACTATCAAAAACCATATCAAAAGCT
TGAAGCTTTTTGTACGGGTTGCTTAAGATCTTGGCTTCTCCTTCTTTTCCTTGAAGAGGG
CCAAGAGTGAAACACCAGAAACCTTCACGACCTTGAACCTAACTCCGGGAATATCTCCCA
CAGCGTGACCCTTACGACCAAATCCAGCAATCAAGACCTCATCCTGCAACACCATAAACA
AGAAATGTATCAGCATATGAATGAAACTAATCCATAGTGTGATGATTGAATAGTCATTTC
ATGTGACTTACGTTTTCCTCGATGTAGTTCAAACAACCATCATTGGGGACAAAGGCAGCA
ATCTTCTTTCCATTCTTGATAAGCTGAACTCTAGCACACTTACGGATAGCAGAGTTAGGC
TGCTTAGCCTCAATACCACTGCATCATCAAACACAAGAGTCTCATCAACGACCAATGTCT
CTACTCAACAAATCAATTATAAGAAAAAGTAAACTTGAATGTTATATCAGACTTACATTT
TCTCAAGAACAATTCCTTTAGCGTGAGAAGAACCAGCAAAAGGCTTCTTCCACTCATTTC
CCAAGTGAGACTTCTTGTACTGCTTGTCAGCCCACCTCTGGTTAATTCGGAGTCTCTTCA
GCTTACGCCCAGCTCCCATACCACGTGTCTTACTGTTAACAAATCATCACAAAATAACAT
TTGAGAACAACTTCACTCGATAAACCATAGTTAAAACTCAGAAAATCAATAAGAAATTCA
TCTAAATAATAACAAGACGATAGAAGATTTATCTCTAATACATCAGCAAAAGTGAGCTTC
AACC

Last Updated on 06.12.2018 16:11