Sequences from confirmation amplicon


Confirmation sequences for line 173E09 and hit At1g69240 are shown.
Line ID 173E09
Locus At1g69240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26028873
Primers used for PCR CN39 - 8474, show primer details
   
Sequence Name 35-K104161-0022-173-E09C-CN39
GenBank Accession LN501813 [GenBank]
Sequencing primer gene specific primer, CN39: 5'-CAGCACTTGCTACACATGGTTAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K104161-0022-173-E09C-CN39
AATTCTCTTTGAGATTGTTTTTTCTATGTCGACACCTATATTGAGGCTTATTATATGATA
TAAATGGATGAAATCAGGAGCTAAAGGTCGAAGGTGCGGAGACAAAGCGGTTCGTGCTGG
TTCATGGAGGAGGGTTTGGTGCATGGTGTTGGTACAAAACCATAACGCTCTTAGAGAAAC
ATGGTTTCCAAGTAGACGCCGTTGACTTGACCGGTTCCGGTGTCAGCTCTTTTGACACAA
ACAACATCACTAGCCTCGCTCAATACGTCAAACCACTCCTCCATTTCTTTGACACCCTTA
AACCCACCGAGAAGGTCTGATCATTTTCATATTATCACAATGTTCGTTTTGGTCTAATTA
TTAATAGTATAAATTATACTTTAATTTACCAACGAGTTTAGGATATAAATTAATTAATGG
GTGTGTGCAGGTGATATTAGTGGGGCATGATTTTGGAGGAGCTTGTATGTCTTATGCCAT
GGAGATGTACCCTTCTTGACTATCATTGATTATTTCCTTTTTTCCAGGATATATTCAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 35-K104162-0022-173-E09C-8409
GenBank Accession LN501814 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K104162-0022-173-E09C-8409
TTCGGACTGAGCCATTTACATTGAATATATCCTGGAAAAAAGGAAATAATCAATGATAGT
CAAGAAGGGTACATCTCCATGGCATAAGACATACAAGCTCCTCCAAAATCATGCCCCACT
AATATCACCTGCACACACCCATTAATTAATTTATATCCTAAACTCGTTGGTAAATTAAAG
TATAATTTATACTATTAATAATTAGACCAAAACGAACATTGTGATAATATGAAAATGATC
AGACCTTCTCGGTGGGTTTAAGGGTGTCAAAGAAATGGAGGAGTGGTTTGACGTATTGAG
CGAGGCTAGTGATGTTGTTTGTGTCAAAAGAGCTGACACCGGAACCGGTCAAGTCAACGG
CGTCTACTTGGAAACCATGTTTCTCTAAGAGCGTTATGGTTTTGTACCAACACCATGCAC
CAAACCCTCCTCCATGAACCAGCACGAACCGCTTTGTCTCCGCACCTTCGACCTTTAGCT
CCTGATTTCATCCATTTATATCAAATAATAAGCCTCAATATAGGTGTCGACATAGAAAAA
ACAATCTCAATAGAGAATTTGGCTAAAACAAAAGATTATTGTAATTAACCATGTGTACAA
AGGTGCT

Last Updated on 06.12.2018 16:11