Sequences from confirmation amplicon


Confirmation sequences for line 173G01 and hit At1g02770 are shown.
Line ID 173G01
Locus At1g02770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:605891
Primers used for PCR BL55 - 8474, show primer details
   
Sequence Name 27-K105062-0022-173-G01C-BL55
GenBank Accession LN498380 [GenBank]
Sequencing primer gene specific primer, BL55: 5'-AGAGGATTTTTACCTTTGGGTTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K105062-0022-173-G01C-BL55
AATTTGTCACAAAGATTAACTTTAATAGATCTGCTTAATCTATGATTGTTCTGTTTTTTT
GAACGAAAATTTCTCTTCTTGCTAAGTGATTTATTATTAATTTTTTCGTAATTAGGGTTT
CGATGTCGAGGGAATCCAAGCACCACCAGGGGGCACTCCATTGATACACTACGACTGTCA
TCTGCCCAATAGTAGGCATCCTGATCCTGTATTGGTCAAGCTTTATGCTAGTGCGGGGCT
TCATCGCTACAATATGTTGGAGGTAAAAAAAACTCCATCGTGGTAACAGAATGCTTGTGT
ACATACATGACATTTAATTGCTCTCTTTTTCTCAGGGGACGAACTTCAAGCTCGTTGATG
TAATGAAATTCAACAAGTTAATGATGCATTTGTCCCCTTTCTACATAACTTTACTTGCAC
AAGATCCAGTTAGCCGTTCGCAGCAAACCTTTCAGGTTCAAGTTGATGAACACTGTCTTA
GCACTATGGATTTGACGGTTCTAATTGCTAGACCTAAAGGTAAGTAAACATTGTTGTTGT
TGTTGTTCGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCCA
   
Sequence Name 11-K105063-0022-173-G01C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K105063-0022-173-G01C-8409
ATATATCCTGGNACGAACAACANNACAACAACAATGGTTTACTNNNTACCTTTA

Last Updated on 06.12.2018 16:11