Sequences from confirmation amplicon


Confirmation sequences for line 174C12 and hit At1g02890 are shown.
Line ID 174C12
Locus At1g02890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:648496
Primers used for PCR MZ84 - 8474, show primer details
   
Sequence Name 43-K026964-022-174-C12C-8409
GenBank Accession LN504711 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K026964-022-174-C12C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGCTAATATCACGAAAATTACCAGTACA
AAAAAAACCATGGTCTTCTTCGCATAGACCACCAAGATCATTGCCATCTGGTATCGATCT
ATCAAATCTAACCCCGATTTTTGAAGATCCATTGCCTTCAAACGCAAGGAGTACTTTTCC
TTGAAAACCTGTTGCCGGTCCCCTAGACAGAATAATACGAAAAAAGAAATAAATTGAGTT
TTGACAACAACAATCTACATCAGATGAAAACCCCTCGAACAAAAGAAGAGAATAAAAACA
AAAAACAATACCTAGGTGGAGCTCGGAGAGAAGCAAGAGAAGAAGTTGAAGGGCCTAAAA
ACCTTACTCGATCACCTAAGAATTACGGAAGTGCTTGTTCAGTAACACAGAAAGTGGCAC
CACAAAGGAAAAAAAACAAGACATAAGCAACCGTACCTGCTTTGAAGGTGTAACTTTTAG
AGGTTGCAGTTGATACTTCTTGCCTCCGAACAGCCTGAGAGCTCAATGTTGATCCACCTG
TTATACCAGCCTCTACACTTGAAATTGGTTTCTTATGCTGCAAAACAGCAGCTTGTGCAG
CTTGTACAGCTCGTTTAGCAAGCACGGACAGTCTTTCACGCCTAGAACTTTCTTT
   
Sequence Name 47-K026964-022-174-C12C-MZ84
GenBank Accession LN506077 [GenBank]
Sequencing primer gene specific primer, MZ84: 5'-CCAAGGAAGCAGATACTACCAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K026964-022-174-C12C-MZ84
GTTCTAGGCGTGAAAGACTGTCCGTGCTTGCTAAACGAGCTGTACAAGCTGCACAAGCTG
CTGTTTTGCAGCATAAGAAACCAATTTCAAGTGTAGAGGCTGGTATAACAGGTGGATCAA
CATTGAGCTCTCAGGCTGTTCGGAGGCAAGAAGTATCAACTGCAACCTCTAAAAGTTACA
CCTTCAAAGCAGGTACGGTTGCTTATGTCTTGTTTTTTTTCCTTTGTGGTGCCACTTTCT
GTGTTACTGAACAAGCACTTCCGTAATTCTTAGGTGATCGAGTAAGGTTTTTAGGCCCTT
CAACTTCTTCTCTTGCTTCTCTCCGAGCTCCACCTAGGTATTGTTTTTTGTTTTTATTCT
CTTCTTTTGTTCGAGGGGTTTTCATCTGATGTAGATTGTTGTTGTCAAAACTCAATTTAT
TTCTTTTTTCGTATTATTCTGTCTAGGGGACCGGCAACAGGTTTTCAAGGAAAAGTACTC
CTTGCGTTTGAAGGCAATGGATCTTCAAAAATCGGGGTTAGATTTGATAGATCGATACCA
GATGGCAATGATCTTGGTGGTCTATGCGAAGAAGACCATGGTTTTTTTTGTACTGGTAAT
TTTCGTGATATTAGCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAA

Last Updated on 06.12.2018 16:11