Sequences from confirmation amplicon


Confirmation sequences for line 174G04 and hit At3g25810 are shown.
Line ID 174G04
Locus At3g25810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9433135
Primers used for PCR 29tg - 8474, show primer details
   
Sequence Name 35-K116785-0022-174-G04C-29tg
GenBank Accession LN501973 [GenBank]
Sequencing primer gene specific primer, 29tg: 5'-AATCATATTCCACACAAGTTCACA-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K116785-0022-174-G04C-29tg
GTTTTTTTTTGTAAAAAAAATTATAGGAAAAATGATATGTTGATGATAGATTATTAGGAT
TTTAATCAGCTATTTTTCATGATATTCTTAGTTTTAAATAATAATGTTATCTAAAATATT
TATTTGCAGTTGGGATATAAATCGTCTTGATGAGCTCCCCGAATACATGAAGTTGTGTTT
TCTTACATTGTTTAATGAAATCAATGCCATGGGATGTGATGTTCTCAAATGTAAAAATAT
TGACGTCATTCCTTATTTCAAAAAATCGGTAAGTTCAAATTTGTATTTACACATTATAGT
TTTATTTTTTGGATAAAAATAAATAATTATTGATTATATATGTTTTTGTTCAAACTATAG
TGGGCAGATTTATGTAAAGCATATCTAGTTGAAGCAAAGTGGTACAAAGGAGGTCAATGT
AGTTTAAACCGAAGATTTTAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCT
GTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGG
GATTGTGCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 16-K116784-0022-174-G04C-8409
GenBank Accession LN493596 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K116784-0022-174-G04C-8409
CACTATCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGG
GTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTAAAATCTTCGGTTTAAACTACA
TTGACCTCCTTTGTACCACTTTGCTTCAACTAGATATGCTTTACATAAATCTGCCCACTA
TAGTTTGAACAAAAACATATATAATCAATAATTATTTATTTTTATCCAAAAAATAAAACT
ATAATGTGTAAATACAAATTTGAACTTACCGATTTTTTGAAATAAGGAATGACGTCAATA
TTTTTACATTTGAGAACATCACATCCCATGGCATTGATTTCATTAAACAATGTAAGAAAA
CACAACTTCATGTATTCGGGGAGCTCATCAAGACGATTTATATCCCAACTGCAAATAAAT
ATTTTAGATAACATTATTATTTAAAACTAAGAATATCATGAAAAATAGCTGATTAAAATC
CTAATAATCTATCATCAACATATCATTTTTCCTATAATTTTTATTTACAAAAAAAAACTA
ATAGAAAGATGGAATTGAAAAAAATATTTATGTGAACTTGTTGAAAA

Last Updated on 06.12.2018 16:11