Sequences from confirmation amplicon


Confirmation sequences for line 175H08 and hit At4g12780 are shown.
Line ID 175H08
Locus At4g12780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7513169
Primers used for PCR 08pq - 8474, show primer details
   
Sequence Name 34-K115290-0022-175-H08C-08pq
GenBank Accession LN501532 [GenBank]
Sequencing primer gene specific primer, 08pq: 5'-CTTGAGCAAGCTCTTAAGACAGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K115290-0022-175-H08C-08pq
GATATAAGACACTGAATAGAGTGCATGCTACAAAGCCAGAGATAAAGGCACTACAATTAT
GAAAGCATGATGTCTCTAAGCTCTATACCCTTTGAGTCATTTCACACAAGCAAGCTCTCA
AATTTAAAGAGAGAAGGTGACAGGAGCAACCCTAACTAGTAACCACAGGTCTAGGAGAAA
CTAATTATTAAGGCAATGACCGTAGCCACAGATCAAAAATGAGATTGTGCATATGAATGA
CATGAATCTTGAAGTTTCTAAAGTTTTTTTTTCCAGATTTCTCACTCAAGTTTTAGAGAG
TATTCATAAAAGAACCAATATCCAAGCTCTGTGAATGGCTTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 10-K115289-0022-175-H08C-8409
GenBank Accession LN490787 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K115289-0022-175-H08C-8409
CATTTCAGCCATTCACAGAGCTTGGATATTGGTTCTTTTATGAATACTCTCTAAAACTTG
AGTGAGAAATCTGGAAAAAAAAACTTTAGAAACTTCAAGATTCATGTCATTCATATGCAC
AATCTCATTTTTGATCTGTGGCTACGGTCATTGCCTTAATAATTAGTTTCTCCTAGACCT
GTGGTTACTAGTTAGGGTTGCTCCTGTCACCTTCTCTCTTTAAATTTGAGAGCTTGCTTG
TGTGAAATGACTCAAAGGGTATAGAGCTTAGAGACATCATGCTTTCATAATTGTAGTGCC
TTTATCTCTGGCTTTGTAGCATGCACTCTATTCAGTGTCTTTATATCCTGACATTGCTCT
TCAAATTTGTTTCCCCAGACCTGTCTTAAGAGCTTGCTCAA

Last Updated on 06.12.2018 16:11