Sequences from confirmation amplicon


Confirmation sequences for line 176A04 and hit At3g46280 are shown.
Line ID 176A04
Locus At3g46280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17008255
Primers used for PCR 06fe - 8474, show primer details
   
Sequence Name 22-K110907-0022-176-A04C-06fe
GenBank Accession LN496165 [GenBank]
Sequencing primer gene specific primer, 06fe: 5'-AGCTGTGCAATCTCAATAAGTCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K110907-0022-176-A04C-06fe
TATGGGTGCATGTGAGTAAGTTAAATTTAGCTCGATGATGATGGAGGCGCTTCGTTATGT
CATCACTCTCTTCTCTCTCACGACCGAAGCGTTACATTAGGAGAAAGTACGTTACAAACA
TTAATATTTTTCTTAATTTGATGACGCAATGAAATTTTATTTTAAGTCTACTATACTTTT
TTTTTTATTTGGCCTATATTCAATTGGAAATGGCTTCATGTCCGGGAAATCTACGTGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCTTTTTTTTTTC
AATTCAAAAATGTAGATGTCCG
   
Sequence Name 06-K110898-0022-176-A04C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K110898-0022-176-A04C-8409
GTGTTTGTGGAGAAAAGGAAAGAAAAACTATTTATTTTTTTTCGCTT

Last Updated on 06.12.2018 16:11