Sequences from confirmation amplicon


Confirmation sequences for line 176D01 and hit At1g70060 are shown.
Line ID 176D01
Locus At1g70060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26385252
Primers used for PCR 17bk - 8474, show primer details
   
Sequence Name 18-K107548-0022-176-D01C-17bk
GenBank Accession LN494333 [GenBank]
Sequencing primer gene specific primer, 17bk: 5'-GAAGTCTCCGATAACTAGGTGTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K107548-0022-176-D01C-17bk
TTCACTGATAGTTAGCCCATTTCTGACTCGCTGCCCCTTTTCAAGCCTCTCCTTCTCACG
GTCTCTTTCTCTATACCTTTCAATCTTCTCACGTTCTCGGTCTGTATCTTTGTCAAGTGA
CTTAGTAGGTTGAGGACATTTGCCTTCACTCCACAAGGATTCTGTAGTTACAGTAATCCA
ATGGTTAGAGTCAATTCAATACGTCCGAAGAGGTATGTCAGAAGTATATTATAAAAGGTA
CTAGACTTACTCTTAGACACGATACCAGAGAGTAATCCATCTGCAGAAAGAAAACAGAAA
AAAGGTCAAATCTTAAGTTCTTTGACTGGCGAGTGTAAGAAGAGAAAGCACTAGTTGGAC
TGGTTATTACCATTCTTGTCACACTGAGCCAAAAAGACTTTGAAGGCGTCCATAAGGTCT
GGATATACACCAATTAAATCACTCACCTAATACAAGAATATATATGTTCACGAGTCATTG
ACAGGTAAAGTCCATAAGGTTTCTCACAAACTTCTCCAACTTAACCATGCAGTGAGAAAG
CTTAAGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCC
   
Sequence Name 02-K107547-0022-176-D01C-8409
GenBank Accession LN487060 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K107547-0022-176-D01C-8409
CATTTACATTGAATTATCCTGACTTAAGCTTTCTCACTGCATGGTTAAGTTGGAGAAGTT
TGTGAGAAACCTTATGGACTTTACCTGTCAATGACTCGTGAACATATATATTCTTGTATT
AGGTGAGTGATTTAATTGGTGTATATCCAGACCTTATGGACGCCTTCAAAGTCTTTTTGG
CTCAGTGTGACAAGAATGGTAATAACCAGTCCAACTAGTGCTTTCTCTTCTTACACTCGC
CAGTCAAAGAACTTAAGATTTGACCTTTTTTCTGTTTTCTTTCTGCAGATGGATTACTCT
CTGGTATCGTGTCTAAGAGTAAGTCTAGTACCTTTTATAATATACTTCTGACATACCTCT
TCGGACGTATTGAATTGACTCTAACCATTGGATTACTGTAACTACAGAATCCTTGTGGAG
TGAAGGCAAATGTCCTCAACCTACTAAGTCACTTGACAAAGATACAGACCGAGAACGTGA
GAAGATTGAAAGGTATAGAGAAAGAGACCGTGAGAAGGAGAGGCTTGAAAAGGTGGCAGC
GAGTCAGAAATGGGCTAAACCTATCAGTGAACTAGATCTCTCGAACTGTGAACAGTGCAC
ACCTAGTTATCGGAAGACTT

Last Updated on 06.12.2018 16:11